BRENDA - Enzyme Database
show all sequences of 2.6.1.9

Imidazolylacetolphosphate:L-glutamate aminotransferase. Purification and physical properties

Martin, R.G.; Goldberger, R.F.; J. Biol. Chem. 242, 1168-1174 (1967)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
equilibrium centrifugation, pyridoxal phosphate analysis, spectrophotometric assay, phosphate analysis, viscometry
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
Salmonella enterica subsp. enterica serovar Typhimurium
eighth step in histidine biosynthesis
L-histidinol phosphate + 2-oxoglutarate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
derepressed histidine auxotroph
-
Purification (Commentary)
Commentary
Organism
from derepressed histidine auxotrophs, 95% pure
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1885
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
eighth step in histidine biosynthesis
637143
Salmonella enterica subsp. enterica serovar Typhimurium
L-histidinol phosphate + 2-oxoglutarate
-
-
-
-
L-histidinol phosphate + 2-oxoglutarate
-
637143
Salmonella enterica subsp. enterica serovar Typhimurium
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
marked tendency to polymerize upon ageing
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
unstable below
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
1 pyridoxal phosphate group associated with each molecule of enzyme; a pyridoxal phosphate protein
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
1 pyridoxal phosphate group associated with each molecule of enzyme; a pyridoxal phosphate protein
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
equilibrium centrifugation, pyridoxal phosphate analysis, spectrophotometric assay, phosphate analysis, viscometry
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
Salmonella enterica subsp. enterica serovar Typhimurium
eighth step in histidine biosynthesis
L-histidinol phosphate + 2-oxoglutarate
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
from derepressed histidine auxotrophs, 95% pure
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1885
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
eighth step in histidine biosynthesis
637143
Salmonella enterica subsp. enterica serovar Typhimurium
L-histidinol phosphate + 2-oxoglutarate
-
-
-
-
L-histidinol phosphate + 2-oxoglutarate
-
637143
Salmonella enterica subsp. enterica serovar Typhimurium
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
marked tendency to polymerize upon ageing
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
-
unstable below
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 2.6.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739670
Nasir
Crystal structures of Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Sci. Rep.
6
18880
2016
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3
3
737370
Nasir
Sample preparation, crystalliz ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
69
445-448
2013
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1
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721229
Nasir
Molecular cloning, overexpress ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. F
68
32-36
2012
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1
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684151
Marienhagen
Insights into the structural b ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. D
64
675-685
2008
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1
4
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15
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15
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676602
Mo
The hpa1 mutant of Arabidopsis ...
Arabidopsis thaliana
Plant Physiol.
141
1425-1435
2006
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659343
Fernandez
Structural studies of the cata ...
Thermotoga maritima
J. Biol. Chem.
279
21478-21488
2004
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1
1
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4
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637153
Mizuguchi
Characterization of histidinol ...
Escherichia coli
Biochim. Biophys. Acta
1647
321-324
2003
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1
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4
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637150
El Malki
Molecular characterization and ...
Nicotiana tabacum
Plant Mol. Biol.
45
191-199
2001
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1
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637152
Haruyama
Structures of Escherichia coli ...
Escherichia coli
Biochemistry
40
4633-4644
2001
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1
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637154
Sivaraman
Crystal structure of histidino ...
Escherichia coli
J. Mol. Biol.
311
761-776
2001
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1
1
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6
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637151
Gu
Imidazole acetol phosphate ami ...
Zymomonas mobilis
J. Bacteriol.
177
1576-1584
1995
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1
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3
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637144
Hsu
L-Histidinol phosphate aminotr ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochimie
71
477-489
1989
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636659
Weigent
Purification and properties of ...
Bacillus subtilis
J. Biol. Chem.
251
6974-6980
1976
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637147
Henderson
Omega-aminoalkylagaroses in th ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
13
4335-4338
1974
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637146
Henderson
Crystalline L-histidinol phosp ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
248
1906-1911
1973
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637149
Roberts
-
Imidazolylacetolphosphate amin ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
248
77408-7753
1973
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637143
Martin
Imidazolylacetolphosphate:L-gl ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
242
1168-1174
1967
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637145
Martin
Imidazolylacetolphosphate:L-gl ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
242
1175-1181
1967
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637142
Ames
Biosynthesis of histidine: imi ...
Neurospora sp.
J. Biol. Chem.
220
113-128
1956
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