Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
equilibrium centrifugation, pyridoxal phosphate analysis, spectrophotometric assay, phosphate analysis, viscometry | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate | Salmonella enterica subsp. enterica serovar Typhimurium | eighth step in histidine biosynthesis | L-histidinol phosphate + 2-oxoglutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
derepressed histidine auxotroph | - |
Purification (Comment) | Organism |
---|---|
from derepressed histidine auxotrophs, 95% pure | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1885 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate | eighth step in histidine biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | L-histidinol phosphate + 2-oxoglutarate | - |
? | |
L-histidinol phosphate + 2-oxoglutarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | marked tendency to polymerize upon ageing | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
unstable below | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Salmonella enterica subsp. enterica serovar Typhimurium | |
pyridoxal 5'-phosphate | 1 pyridoxal phosphate group associated with each molecule of enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |