General Stability | Organism |
---|---|
pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay | Nicotiana sylvestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
prephenate | substrate inhibition above 1 mM, antagonism by L-glutamate | Nicotiana sylvestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + L-glutamate | Nicotiana sylvestris | important step in biosynthesis of L-tyrosine and L-phenylalanine | L-arogenate + 2-oxoglutarate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana sylvestris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially by ion exchange chromatography | Nicotiana sylvestris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | stable cell line ANS-1 | Nicotiana sylvestris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Nicotiana sylvestris |
0.042 | - |
crude enzyme extract, substrates prephenate and L-glutamate | Nicotiana sylvestris |
0.073 | - |
partially purified enzyme, substrate pyruvate and L-glutamate | Nicotiana sylvestris |
0.077 | - |
partially purified enzyme, substrate prephenate and L-aspartate | Nicotiana sylvestris |
0.118 | - |
partially purified enzyme, substrate oxaloacetate and L-aspartate | Nicotiana sylvestris |
0.125 | - |
partially purified enzyme, substrate oxaloacetate and L-glutamate | Nicotiana sylvestris |
0.156 | - |
partially purified enzyme, substrate 2-oxoglutarate and L-aspartate | Nicotiana sylvestris |
0.157 | - |
partially purified enzyme, substrate prephenate and L-glutamate | Nicotiana sylvestris |
0.251 | - |
partially purified enzyme, substrate 2-oxoglutarate and L-glutamate | Nicotiana sylvestris |
Storage Stability | Organism |
---|---|
in presence of pyridoxal 5'-phosphate, EDTA, glycerol, stable for at least one month | Nicotiana sylvestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + L-aspartate | - |
Nicotiana sylvestris | L-glutamate + oxaloacetate | - |
r | |
2-oxoglutarate + L-glutamate | - |
Nicotiana sylvestris | L-glutamate + 2-oxoglutarate | - |
r | |
additional information | substrate specificity, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and indolepyruvate using cosubstrate L-glutamate, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and pyruvate using cosubstrate L-aspartate | Nicotiana sylvestris | ? | - |
? | |
oxaloacetate + L-aspartate | - |
Nicotiana sylvestris | L-aspartate + oxaloacetate | - |
r | |
oxaloacetate + L-glutamate | - |
Nicotiana sylvestris | L-aspartate + 2-oxoglutarate | - |
r | |
prephenate + L-aspartate | - |
Nicotiana sylvestris | L-arogenate + oxaloacetate | - |
r | |
prephenate + L-glutamate | important step in biosynthesis of L-tyrosine and L-phenylalanine | Nicotiana sylvestris | L-arogenate + 2-oxoglutarate | - |
r | |
prephenate + L-glutamate | optimal prephenate concentration is 0.5-1.0 mM | Nicotiana sylvestris | L-arogenate + 2-oxoglutarate | - |
r | |
pyruvate + L-glutamate | - |
Nicotiana sylvestris | L-alanine + 2-oxoglutarate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AT-C | - |
Nicotiana sylvestris |
prephenate aminotransferase | - |
Nicotiana sylvestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Nicotiana sylvestris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 70 | - |
Nicotiana sylvestris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable, at least for 17 min | Nicotiana sylvestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.25 | - |
- |
Nicotiana sylvestris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required, stabilizes the enzyme | Nicotiana sylvestris |