BRENDA - Enzyme Database
show all sequences of 2.6.1.73

Purification and characterization of methionine:glyoxylate aminotransferase from Brassica carinata and Brassica napus

Chapple, C.C.S.; Glover, J.R.; Ellis, B.E.; Plant Physiol. 94, 1887-1896 (1990)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
x * 49000, SDS-PAGE
Brassica carinata
51000
-
x * 51000, SDS-PAGE
Brassica napus
230000
290000
gel filtration
Brassica napus
230000
290000
gel filtration
Brassica carinata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Brassica napus
function in the deamination of methionine or production of methionine in the methionine-cycle
?
-
-
?
additional information
Brassica carinata
function in the deamination of methionine or production of methionine in the methionine-cycle
?
-
-
?
additional information
Brassica carinata
involved in the biosynthesis of methionine-derived glucosinolates
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Brassica carinata
-
var. R-4218
-
Brassica napus
-
var. Topas
-
Purification (Commentary)
Purification (Commentary)
Organism
purification on Mono Q results in isolation of three isoenzymes, MGAT 1, MGAT 2 and MGAT 3
Brassica carinata
var. R-4218, partial
Brassica carinata
var. Topas, partial
Brassica napus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Brassica napus
-
leaf
-
Brassica carinata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.06
-
isoenzyme MGAT 3, 30°C, pH 8.2
Brassica carinata
0.066
-
MGAT, single enzyme 30°C, pH 8.2
Brassica napus
0.18
-
isoenzyme MGAT 2 30°C, pH 8.2
Brassica carinata
0.6
-
isoenzyme MGAT 1, overall activity without separation of isoenzymes 30°C, pH 8.2
Brassica carinata
Storage Stability
Storage Stability
Organism
-60°C, desalted enzyme preparation
Brassica napus
-60°C, desalted enzyme preparation
Brassica carinata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
636990
Brassica napus
2-oxoglutarate + L-methionine
-
-
-
?
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
636990
Brassica carinata
2-oxoglutarate + L-methionine
-
-
-
?
L-glutamine + glyoxylate
-
636990
Brassica napus
2-oxoglutarate + glycine
-
-
-
?
L-glutamine + glyoxylate
-
636990
Brassica carinata
2-oxoglutarate + glycine
-
-
-
?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
-
636990
Brassica napus
4-methylsulfanyl-2-oxobutanoate + L-methionine
-
-
-
?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
-
636990
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-methionine
-
-
-
?
L-methionine + glyoxylate
-
636990
Brassica napus
4-methylsulfanyl-2-oxobutanoate + glycine
-
-
-
?
L-methionine + glyoxylate
-
636990
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + glycine
-
-
-
?
L-methionine + oxaloacetate
24% of activity compared to glyoxylate
636990
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-aspartate
-
-
-
?
additional information
function in the deamination of methionine or production of methionine in the methionine-cycle
636990
Brassica napus
?
-
-
-
?
additional information
function in the deamination of methionine or production of methionine in the methionine-cycle
636990
Brassica carinata
?
-
-
-
?
additional information
involved in the biosynthesis of methionine-derived glucosinolates
636990
Brassica carinata
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 51000, SDS-PAGE
Brassica napus
?
x * 49000, SDS-PAGE
Brassica carinata
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Brassica napus
30
-
assay at
Brassica carinata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
49000
-
x * 49000, SDS-PAGE
Brassica carinata
51000
-
x * 51000, SDS-PAGE
Brassica napus
230000
290000
gel filtration
Brassica napus
230000
290000
gel filtration
Brassica carinata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Brassica napus
function in the deamination of methionine or production of methionine in the methionine-cycle
?
-
-
?
additional information
Brassica carinata
function in the deamination of methionine or production of methionine in the methionine-cycle
?
-
-
?
additional information
Brassica carinata
involved in the biosynthesis of methionine-derived glucosinolates
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
purification on Mono Q results in isolation of three isoenzymes, MGAT 1, MGAT 2 and MGAT 3
Brassica carinata
var. R-4218, partial
Brassica carinata
var. Topas, partial
Brassica napus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Brassica napus
-
leaf
-
Brassica carinata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.06
-
isoenzyme MGAT 3, 30°C, pH 8.2
Brassica carinata
0.066
-
MGAT, single enzyme 30°C, pH 8.2
Brassica napus
0.18
-
isoenzyme MGAT 2 30°C, pH 8.2
Brassica carinata
0.6
-
isoenzyme MGAT 1, overall activity without separation of isoenzymes 30°C, pH 8.2
Brassica carinata
Storage Stability (protein specific)
Storage Stability
Organism
-60°C, desalted enzyme preparation
Brassica napus
-60°C, desalted enzyme preparation
Brassica carinata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
636990
Brassica napus
2-oxoglutarate + L-methionine
-
-
-
?
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
-
636990
Brassica carinata
2-oxoglutarate + L-methionine
-
-
-
?
L-glutamine + glyoxylate
-
636990
Brassica napus
2-oxoglutarate + glycine
-
-
-
?
L-glutamine + glyoxylate
-
636990
Brassica carinata
2-oxoglutarate + glycine
-
-
-
?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
-
636990
Brassica napus
4-methylsulfanyl-2-oxobutanoate + L-methionine
-
-
-
?
L-methionine + 4-methylsulfanyl-2-oxobutanoate
-
636990
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-methionine
-
-
-
?
L-methionine + glyoxylate
-
636990
Brassica napus
4-methylsulfanyl-2-oxobutanoate + glycine
-
-
-
?
L-methionine + glyoxylate
-
636990
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + glycine
-
-
-
?
L-methionine + oxaloacetate
24% of activity compared to glyoxylate
636990
Brassica carinata
4-methylsulfanyl-2-oxobutanoate + L-aspartate
-
-
-
?
additional information
function in the deamination of methionine or production of methionine in the methionine-cycle
636990
Brassica napus
?
-
-
-
?
additional information
function in the deamination of methionine or production of methionine in the methionine-cycle
636990
Brassica carinata
?
-
-
-
?
additional information
involved in the biosynthesis of methionine-derived glucosinolates
636990
Brassica carinata
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 51000, SDS-PAGE
Brassica napus
?
x * 49000, SDS-PAGE
Brassica carinata
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Brassica napus
30
-
assay at
Brassica carinata
Other publictions for EC 2.6.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
636990
Chapple
Purification and characterizat ...
Brassica carinata, Brassica napus
Plant Physiol.
94
1887-1896
1990
-
-
-
-
-
-
-
-
-
-
4
3
-
2
-
-
3
-
-
2
4
2
12
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
3
-
-
-
3
-
2
4
2
12
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
636989
Glover
-
Allylglucosinolate biosynthesi ...
Brassica carinata
Phytochemistry
27
1345-1348
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
14
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
14
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-