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Literature summary for extracted from

  • Breen, R.S.; Campopiano, D.J.; Webster, S.; Brunton, M.; Watt, R.; Baxter, R.L.
    The mechanism of 7,8-diaminopelargonate synthase; the role of S-adenosylmethionine as the amino donor (2003), Org. Biomol. Chem., 1, 3498-3499.
    View publication on PubMed


Cloned (Comment) Organism
cloning from strain JM101, overexpression in strain BL21(DE3) Escherichia coli


Inhibitors Comment Organism Structure
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
additional information kinetic analysis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate Escherichia coli enzyme is obligate catalyzing the second step in the biosynthesis of vitamin H, i.e. biotin S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate


Organism UniProt Comment Textmining
Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from strain BL21(DE3) Escherichia coli


Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate ping pong kinetics, reaction mechanism, via a enzyme-pyridoxamine phosphate, a quinonoid intermediate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate enzyme is obligate catalyzing the second step in the biosynthesis of vitamin H, i.e. biotin Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
S-adenosyl-L-methionine + 8-amino-7-oxononanoate stereospecific amination Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate


Synonyms Comment Organism
7,8-diaminopelargonate synthase
Escherichia coli
DAPA synthase
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
assay at Escherichia coli


Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Escherichia coli