Crystallization (Comment) | Organism |
---|---|
- |
Achromobacter superficialis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
156000 | - |
sedimentation equilibrium | Achromobacter superficialis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter superficialis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, Sephadex G-150, hydroxylapatite, ammonium sulfate, Sephadex g-150, crystalization | Achromobacter superficialis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.19 | - |
- |
Achromobacter superficialis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-aminopropanesulfonate + 2-oxoglutarate | - |
Achromobacter superficialis | 2-oxopropanesulfonate + L-glutamate | - |
? | |
beta-alanine + 2-oxoglutarate | - |
Achromobacter superficialis | 3-oxopropanoate + L-glutamate | - |
? | |
DL-3-aminoisobutyrate + 2-oxoglutarate | - |
Achromobacter superficialis | 2-methyl-3-oxopropanoate + L-glutamate | - |
? | |
taurine + 2-oxoglutarate | no activity with pyruvate, phenylpyruvate, oxaloacetate | Achromobacter superficialis | sulfoacetaldehyde + L-glutamate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Achromobacter superficialis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Achromobacter superficialis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal phosphate protein | Achromobacter superficialis | |
pyridoxal 5'-phosphate | 4 mol of pyridoxal phosphate per mol of enzyme | Achromobacter superficialis | |
pyridoxal 5'-phosphate | enzyme inactivated by treatment with ammonium sulfate is activated by incubation with pyridoxal 5'-phosphate at 45-60°C for about 10 min | Achromobacter superficialis |