KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
pyruvate | pH 7.0, 37°C, tyrosine as co-substrate | Trypanosoma cruzi | |
6.8 | - |
L-tyrosine | pH 7.0, 37°C, pyruvate as co-substrate | Trypanosoma cruzi | |
16 | - |
oxaloacetate | pH 7.0, 37°C, tyrosine as co-substrate | Trypanosoma cruzi | |
17.9 | - |
L-phenylalanine | pH 7.0, 37°C, pyruvate as co-substrate | Trypanosoma cruzi | |
21.4 | - |
tryptophan | pH 7.0, 37°C, pyruvate as co-substrate | Trypanosoma cruzi | |
38 | - |
2-oxoglutarate | pH 7.0, 37°C, tyrosine as co-substrate | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Trypanosoma cruzi |
91000 | - |
gel filtration | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
epimastigote | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | not a glycoprotein | Trypanosoma cruzi |
Purification (Comment) | Organism |
---|---|
partial, using chromatography on DEAE-cellulose and gel filtration | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.06 | - |
- |
Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + oxaloacetate | - |
Trypanosoma cruzi | L-aspartate + 2-oxoglutarate | - |
? | |
L-glutamate + pyruvate | - |
Trypanosoma cruzi | L-alanine + 2-oxoglutarate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Trypanosoma cruzi | L-glutamate + phenylpyruvate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Trypanosoma cruzi | 4-hydroxyphenylpyruvate + L-glutamate | - |
? | |
tryptophan + 2-oxoglutarate | - |
Trypanosoma cruzi | 3-indole-2-oxopropanoate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Trypanosoma cruzi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
complete inactivation after 5 min | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Trypanosoma cruzi |