Literature summary for 2.6.1.48 extracted from

Kim, H.T.; Khang, T.U.; Baritugo, K.A.; Hyun, S.M.; Kang, K.H.; Jung, S.H.; Song, B.K.; Park, K.; Oh, M.K.; Kim, G.B.; Kim, H.U.; Lee, S.Y.; Park, S.J.; Joo, J.C.
Metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical (2019), Metab. Eng., 51, 99-109 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene davT, recombinant expression of N-terminal His6-tagged enzyme in Corynebacterium glutamicum strain KCTC 1857, coexpression with glutarate semialdehyde dehydrogenase (davD, EC 1.2.1.20) from Pseudomonas putida, 4-aminobutyrate-2-oxoglutarate transaminase (gabT, EC 2.6.1.19) from Corynebacterium glutamicum, and NADP-dependent succinic semialdehyde dehydrogenase (gabD, EC 1.2.1.79) from Corynebacterium glutamicum, as well as N-terminal His6-tagged lysine 2-monooxygenase (davB, EC 1.13.12.2) from Pseudomonas putida	Pseudomonas putida
gene dayB, recombinant expression in Corynebacterium glutamicum, co-expression with Pseudomonas putida dayA and dayB genes encoding encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively, and Corynebacterium glutamicum gabD gene encoding glutarate semialdehyde dehydrogenase	Corynebacterium glutamicum
gene dayT, recombinant expression in Corynebacterium glutamicum, co-expression with Pseudomonas putida dayA, dayB, and dayD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical by co-expression with Pseudomonas putida dayA, dayB, and dayD genes encoding encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively, in Corynebacterium glutamicum. Method optimization and evaluation	Corynebacterium glutamicum
additional information	metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical by co-expression with Pseudomonas putida dayA, dayB, and dayD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively, in Corynebacterium glutamicum. Method optimization and evaluation	Pseudomonas putida
additional information	metabolic engineering of Corynebacterium glutamicum for the production of glutaric acid, a C5 dicarboxylic acid platform chemical, by co-expression of Pseudomonas putida davT, davB, and davD genes encoding lysine 2-monooxygenase, delta-aminovaleramidase, and glutarate semialdehyde dehydrogenase, respectively, in Corynebacterium glutamicum. Method optimization and evaluation. The glutaric acid biosynthesis pathway constructed in recombinant Corynebacterium glutamicum is engineered by examining strong synthetic promoters H30 and H36, Corynebacterium glutamicum codon-optimized davTDBA genes, and modification of davB gene with an N-terminal His6-tag to improve the production of glutaric acid. The use of N-terminal His6-tagged DavB is most suitable for the production of glutaric acid from glucose. Fed-batch fermentation on of the final engineered Corynebacterium glutamicum H30_GAHis strain, expressing davTDA genes along with davB fused with His6-tag at N-terminus can produce 24.5 g/l of glutaric acid with low accumulation of L-lysine (1.7 g/l), wherein 5-aminovaleric acid (5-AVA) ccumulation is not observed during fermentation. Metabolically engineered Corynebacterium glutamicum strain KCTC H30_GA-2 (engineered strain KCTC 1857) is able for catalysis of the biosynthesis of glutaric acid from glucose. Method optimization and evaluation, overview	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5-aminopentanoate + 2-oxoglutarate	Pseudomonas putida	-	5-oxopentanoate + L-glutamate	-	?
5-aminopentanoate + 2-oxoglutarate	Pseudomonas putida DSM 6125	-	5-oxopentanoate + L-glutamate	-	?
5-aminopentanoate + 2-oxoglutarate	Pseudomonas putida NCIMB 11950	-	5-oxopentanoate + L-glutamate	-	?
5-aminopentanoate + 2-oxoglutarate	Pseudomonas putida ATCC 47054	-	5-oxopentanoate + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	A0A0U4XQS6	-	-
Pseudomonas putida	-	-	-
Pseudomonas putida	Q88RB9	-	-
Pseudomonas putida ATCC 47054	Q88RB9	-	-
Pseudomonas putida DSM 6125	Q88RB9	-	-
Pseudomonas putida NCIMB 11950	Q88RB9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-aminopentanoate + 2-oxoglutarate	-	Pseudomonas putida	5-oxopentanoate + L-glutamate	-	?
5-aminopentanoate + 2-oxoglutarate	-	Pseudomonas putida DSM 6125	5-oxopentanoate + L-glutamate	-	?
5-aminopentanoate + 2-oxoglutarate	-	Pseudomonas putida NCIMB 11950	5-oxopentanoate + L-glutamate	-	?
5-aminopentanoate + 2-oxoglutarate	-	Pseudomonas putida ATCC 47054	5-oxopentanoate + L-glutamate	-	?

Synonyms

Synonyms	Comment	Organism
5-aminovalerate transaminase	-	Pseudomonas putida
5-aminovalerate transaminase	-	Corynebacterium glutamicum
davT	-	Pseudomonas putida
dayT	-	Pseudomonas putida
GabT	-	Corynebacterium glutamicum

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Pseudomonas putida

General Information

General Information	Comment	Organism
metabolism	in the L-lysine catabolism pathway, 5-aminovaleric acid (5-AVA) is furter converted into glutarate semialdehyde by 5-aminovalerate transaminase encoded by davT	Pseudomonas putida

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Release 2023.1 (January 2023)