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Literature summary for 2.6.1.45 extracted from

  • Nakamura, Y.; Tolbert, N.E.
    Serine:glyoxylate, alanine:glyoxylate, and glutamate:glyoxylate aminotransferase reactions in peroxisomes from spinach leaves (1983), J. Biol. Chem., 258, 2763-2768.
No PubMed abstract available
Search for more literature for 2.6.1.45

Inhibitors

Inhibitors Comment Organism Structure
glycine competitive vs. L-serine Spinacia oleracea
hydroxylamine 0.002 mM, 92% inhibition, serine, L-alanine and glycine protect Spinacia oleracea
L-alanine competitive vs. L-serine Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 glyoxylate pH 7.0, 27°C Spinacia oleracea
2.7
-
 L-serine pH 7.0, 27°C Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Spinacia oleracea 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + glyoxylate Spinacia oleracea physiologically irreversible 3-hydroxypyruvate + glycine
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach
-

Purification (Commentary)

Purification (Comment) Organism
partial Spinacia oleracea

Reaction

Reaction Comment Organism Reaction ID
L-serine + glyoxylate = 3-hydroxypyruvate + glycine ping-pong reaction mechanism Spinacia oleracea
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.18
-
-
 Spinacia oleracea

Storage Stability

Storage Stability Organism
-18°C, 2 weeks Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + glyoxylate poor substrate Spinacia oleracea pyruvate + glycine
-
 ?
L-serine + glyoxylate
-
 Spinacia oleracea 3-hydroxypyruvate + glycine
-
 r
L-serine + glyoxylate physiologically irreversible Spinacia oleracea 3-hydroxypyruvate + glycine
-
 ?
L-serine + pyruvate poor substrate, 10% of glyoxylate transamination activity Spinacia oleracea 3-hydroxypyruvate + L-alanine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
6 9 approx. 60% of maximal activity at pH 6, approx. half-maximal activity at pH 9 Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required for activity Spinacia oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
33
-
 glycine pH 7.0, 27°C Spinacia oleracea
45
-
 L-alanine pH 7.0, 27°C Spinacia oleracea