BRENDA - Enzyme Database
show all sequences of 2.6.1.45

Serine:glyoxylate, alanine:glyoxylate, and glutamate:glyoxylate aminotransferase reactions in peroxisomes from spinach leaves

Nakamura, Y.; Tolbert, N.E.; J. Biol. Chem. 258, 2763-2768 (1983)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
glycine
competitive vs. L-serine
Spinacia oleracea
hydroxylamine
0.002 mM, 92% inhibition, serine, L-alanine and glycine protect
Spinacia oleracea
L-alanine
competitive vs. L-serine
Spinacia oleracea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
glyoxylate
pH 7.0, 27°C
Spinacia oleracea
2.7
-
L-serine
pH 7.0, 27°C
Spinacia oleracea
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
peroxisome
-
Spinacia oleracea
5777
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-serine + glyoxylate
Spinacia oleracea
physiologically irreversible
3-hydroxypyruvate + glycine
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
spinach
-
Purification (Commentary)
Commentary
Organism
partial
Spinacia oleracea
Reaction
Reaction
Commentary
Organism
L-serine + glyoxylate = 3-hydroxypyruvate + glycine
ping-pong reaction mechanism
Spinacia oleracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.18
-
-
Spinacia oleracea
Storage Stability
Storage Stability
Organism
-18°C, 2 weeks
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-alanine + glyoxylate
poor substrate
636690
Spinacia oleracea
pyruvate + glycine
-
636690
Spinacia oleracea
?
L-serine + glyoxylate
-
636690
Spinacia oleracea
3-hydroxypyruvate + glycine
-
636690
Spinacia oleracea
r
L-serine + glyoxylate
physiologically irreversible
636690
Spinacia oleracea
3-hydroxypyruvate + glycine
-
-
-
-
L-serine + pyruvate
poor substrate, 10% of glyoxylate transamination activity
636690
Spinacia oleracea
3-hydroxypyruvate + L-alanine
-
636690
Spinacia oleracea
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Spinacia oleracea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Spinacia oleracea
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
approx. 60% of maximal activity at pH 6, approx. half-maximal activity at pH 9
Spinacia oleracea
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required for activity
Spinacia oleracea
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
33
-
glycine
pH 7.0, 27°C
Spinacia oleracea
45
-
L-alanine
pH 7.0, 27°C
Spinacia oleracea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
required for activity
Spinacia oleracea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glycine
competitive vs. L-serine
Spinacia oleracea
hydroxylamine
0.002 mM, 92% inhibition, serine, L-alanine and glycine protect
Spinacia oleracea
L-alanine
competitive vs. L-serine
Spinacia oleracea
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
33
-
glycine
pH 7.0, 27°C
Spinacia oleracea
45
-
L-alanine
pH 7.0, 27°C
Spinacia oleracea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
glyoxylate
pH 7.0, 27°C
Spinacia oleracea
2.7
-
L-serine
pH 7.0, 27°C
Spinacia oleracea
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
peroxisome
-
Spinacia oleracea
5777
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-serine + glyoxylate
Spinacia oleracea
physiologically irreversible
3-hydroxypyruvate + glycine
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Spinacia oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.18
-
-
Spinacia oleracea
Storage Stability (protein specific)
Storage Stability
Organism
-18°C, 2 weeks
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-alanine + glyoxylate
poor substrate
636690
Spinacia oleracea
pyruvate + glycine
-
636690
Spinacia oleracea
?
L-serine + glyoxylate
-
636690
Spinacia oleracea
3-hydroxypyruvate + glycine
-
636690
Spinacia oleracea
r
L-serine + glyoxylate
physiologically irreversible
636690
Spinacia oleracea
3-hydroxypyruvate + glycine
-
-
-
-
L-serine + pyruvate
poor substrate, 10% of glyoxylate transamination activity
636690
Spinacia oleracea
3-hydroxypyruvate + L-alanine
-
636690
Spinacia oleracea
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
-
assay at
Spinacia oleracea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Spinacia oleracea
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
approx. 60% of maximal activity at pH 6, approx. half-maximal activity at pH 9
Spinacia oleracea
Other publictions for EC 2.6.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738913
Zhang
Distinct photorespiratory reac ...
Oryza sativa Japonica Group
J. Photochem. Photobiol. B
142
110-117
2015
-
-
1
-
-
-
-
6
-
-
2
-
-
1
-
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1
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3
1
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3
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1
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6
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2
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1
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3
1
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-
3
-
-
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-
-
1
1
-
-
-
723373
Zhang
Characterization of Arabidopsi ...
Arabidopsis thaliana
Phytochemistry
85
30-35
2013
-
-
1
-
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-
8
-
-
-
-
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6
-
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8
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1
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8
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8
-
-
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-
-
-
-
-
1
-
-
1
-
-
723401
Yang
-
Overexpression of the Arabidop ...
Arabidopsis thaliana
Plant Cell Tissue Organ Cult.
113
407-416
2013
-
1
-
-
-
-
-
-
-
-
-
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-
1
-
-
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1
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-
-
1
1
-
-
-
723450
Li
-
Variations in SGAT enzyme acti ...
Chlamydomonas reinhardtii
Plant Physiol. Commun.
46
359-364
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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2
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-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
-
-
702425
Karsten
Detection of a gem-diamine and ...
Hyphomicrobium methylovorum
Biochim. Biophys. Acta
1790
575-580
2009
-
-
1
-
-
-
1
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
1
-
-
-
-
1
1
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-
1
1
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1
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1
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-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702537
Nagata
Assay of alanine:glyoxylate am ...
Homo sapiens
Biomed. Res.
30
295-301
2009
-
-
-
-
-
-
1
-
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2
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2
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1
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1
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1
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1
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2
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1
-
-
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-
684122
Kendziorek
Properties of serine:glyoxylat ...
Arabidopsis thaliana
Acta Biochim. Biophys. Sin. (Shanghai)
40
102-110
2008
-
-
-
-
-
-
3
10
-
-
2
-
-
4
-
-
1
-
-
1
-
-
2
1
-
-
-
2
1
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3
-
10
-
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2
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1
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1
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-
2
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
676345
Tian
-
Synthesis of the photorespirat ...
Chlamydomonas reinhardtii, Chlamydomonas reinhardtii 325
Physiol. Plant.
127
571-582
2006
-
-
-
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1
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2
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2
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1
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2
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657439
Truszkiewicz
Some structural properties of ...
Triticum aestivum, Zea mays
Acta Biochim. Pol.
52
527-534
2005
-
-
-
-
-
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2
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5
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5
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2
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3
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5
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2
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3
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3
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672002
Karsten
Reaction of serine-glyoxylate ...
Hyphomicrobium methylovorum
Biochemistry
44
15930-15936
2005
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1
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2
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1
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1
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2
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660507
Zhu
-
Determination of serine:glyoxy ...
Lemna gibba
Sepu
21
584-586
2003
-
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1
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1
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636702
Karsten
Detection of intermediates in ...
Hyphomicrobium methylovorum
Methods Enzymol.
354
223-237
2002
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1
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1
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1
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1
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1
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1
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636701
Karsten
Initial velocity, spectral, an ...
Hyphomicrobium methylovorum
Arch. Biochem. Biophys.
388
267-275
2001
-
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2
2
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2
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1
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3
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2
2
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3
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636700
Wingler
-
Serine: glyoxylate aminotransf ...
Hordeum vulgare
J. Exp. Bot.
50
719-722
1999
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1
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286510
Hagishita
Immunological characterization ...
Hyphomicrobium methylovorum, Hyphomicrobium sp., Methylobacterium organophilum, Methylobacterium organophilum XX, Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
FEMS Microbiol. Lett.
142
49-52
1996
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25
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6
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6
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636698
Hagishita
Cloning and expression of the ...
Hyphomicrobium methylovorum
Eur. J. Biochem.
241
1-5
1996
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1
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2
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1
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11
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1
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1
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1
1
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1
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1
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1
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-
636697
Paszkowski
-
The hydrosulfide groups of glu ...
Secale cereale
Acta Physiol. Plant.
17
85-90
1995
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3
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1
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636696
Paszkowski
-
Inhibition of glutamate: glyox ...
Secale cereale
Acta Physiol. Plant.
16
217-223
1994
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3
1
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1
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1
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1
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1
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636694
Paszkowski
Some properties of serine: gly ...
Secale cereale
Acta Biochim. Pol.
38
437-448
1991
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7
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1
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1
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1
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3
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1
2
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1
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-
-
7
2
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636693
Izumi
Purification and characterizat ...
Hyphomicrobium methylovorum
Eur. J. Biochem.
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285-290
1990
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-
-
-
-
-
14
2
1
-
3
1
-
3
-
-
1
1
-
-
1
1
8
1
1
-
5
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
14
-
2
1
-
3
1
-
-
-
1
-
-
1
1
8
1
1
-
5
-
2
-
1
-
-
-
-
-
-
-
636695
Paszkowski
Serine:glyoxylate aminotransfe ...
Secale cereale
Acta Biochim. Pol.
37
277-282
1990
-
-
-
-
-
1
-
-
-
-
2
-
-
2
-
-
1
-
-
2
1
-
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
1
-
2
1
-
5
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
636692
Havir
Inactivation of serine:glyoxyl ...
Nicotiana tabacum
Plant Physiol.
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473-478
1986
-
-
-
-
-
1
3
-
-
-
-
-
-
2
-
-
1
-
-
1
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
1
-
1
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636691
Hondred
Isolation of serine:glyoxylate ...
Cucumis sativus
Plant Physiol.
79
95-102
1985
-
-
-
-
-
-
-
-
2
-
3
1
-
2
-
-
1
-
-
3
1
-
3
1
-
-
-
-
-
-
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-
-
-
-
-
-
2
-
3
1
-
-
-
1
-
3
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636689
Ireland
Purification and properties of ...
Pisum sativum
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291-296
1983
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-
-
-
-
-
6
7
1
-
1
1
-
2
-
-
1
-
-
1
1
1
12
-
1
-
-
-
1
1
-
1
4
-
-
-
-
-
1
-
-
-
-
6
4
7
1
-
1
1
-
-
-
1
-
1
1
1
12
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
636690
Nakamura
-
Serine:glyoxylate, alanine:gly ...
Spinacia oleracea
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258
2763-2768
1983
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-
-
-
-
-
3
2
1
-
-
1
-
1
-
-
1
1
-
1
1
1
4
-
1
-
-
-
1
1
-
1
2
-
-
-
-
-
1
-
-
-
-
3
2
2
1
-
-
1
-
-
-
1
-
1
1
1
4
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
636688
Carpe
Serine-glyoxylate aminotransfe ...
Phaseolus vulgaris
Biochim. Biophys. Acta
370
96-101
1974
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-
-
-
-
-
4
4
-
-
-
1
-
2
-
-
1
-
-
2
-
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4
-
1
-
-
-
2
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-
-
-
-
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4
-
4
-
-
-
1
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-
1
-
2
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
636687
Smith
Purification and characterizat ...
Phaseolus vulgaris
Biochim. Biophys. Acta
321
156-164
1973
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-
-
-
-
-
6
4
1
-
-
1
-
2
-
-
1
1
-
2
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
4
1
-
-
1
-
-
-
1
-
2
1
-
3
-
1
-
-
-
1
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-