Cloned (Comment) | Organism |
---|---|
GST-tagged protein expressed in Escherichia coli BL21(DE3) | Streptococcus mutans |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method with PEG 3350 as the primary precipitant | Streptococcus mutans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38140 | - |
monomer with an additional five-amino-acid tag (GPLGS)MALDI-TOF-TOF | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | Q8DTW7 | - |
- |
Streptococcus mutans UA159 | Q8DTW7 | - |
- |
Purification (Comment) | Organism |
---|---|
GST-affinity chromatography, cleavage of the GST tag, anion exchange chromatography, gel filtration | Streptococcus mutans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.74 | - |
glycine, pH 8.0, 3°C | Streptococcus mutans |
5.23 | - |
L-tryptophan, pH 8.0, 3°C | Streptococcus mutans |
77.03 | - |
L-valine, pH 8.0, 3°C | Streptococcus mutans |
86.84 | - |
L-leucine, pH 8.0, 3°C | Streptococcus mutans |
104.6 | - |
L-isoleucine, pH 8.0, 3°C | Streptococcus mutans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + 2-oxoglutarate | - |
Streptococcus mutans | L-glutamic acid + glyoxylate | - |
? | |
glycine + 2-oxoglutarate | - |
Streptococcus mutans UA159 | L-glutamic acid + glyoxylate | - |
? | |
L-isoleucine + 2-oxoglutarate | - |
Streptococcus mutans | 3-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
L-isoleucine + 2-oxoglutarate | - |
Streptococcus mutans UA159 | 3-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Streptococcus mutans | 4-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Streptococcus mutans UA159 | 4-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
L-tryptophan + 2-oxoglutarate | - |
Streptococcus mutans | L-glutamic acid + 3-indole-2-oxopropanoate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Streptococcus mutans UA159 | L-glutamic acid + 3-indole-2-oxopropanoate | - |
? | |
L-valine + 2-oxoglutarate | - |
Streptococcus mutans | 3-methyl-2-oxobutanoic acid + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Streptococcus mutans UA159 | 3-methyl-2-oxobutanoic acid + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
branched-chain aminotransferase | - |
Streptococcus mutans |