Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
isomerase domain of the human GFAT in the presence of cyclic glucose-6-phosphate and linear D-glucosamine 6-phosphate, hanging drop vapor diffusion method, at 20°C using 12% (v/v) isopropanol, 0.8 M ammonium acetate and 40 mM Tris-HCl at pH 8.5 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | Homo sapiens | - |
L-glutamate + D-glucosamine 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q06210 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + D-fructose 6-phosphate | - |
Homo sapiens | L-glutamate + D-glucosamine 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GFAT | - |
Homo sapiens |
glutamine:fructose-6-phosphate amidotransferase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | glutamine:fructose-6-phosphate amidotransferase is a rate-limiting enzyme in the hexoamine biosynthetic pathway and plays an important role in type 2 diabetes | Homo sapiens |