Crystallization (Comment) | Organism |
---|---|
structures of BioU in the resting state, the BioU-conjugate with 8-amino-7-oxononanoate and K124A mutant complexed with NAD+, by molecular replacement | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
D235N | residues His233 and Asp235 are responsible for carboxylation of the N7 atom of the BioU conjugate with 8-amino-7-oxononanoate | Synechocystis sp. |
H233A | residues His233 and Asp235 are responsible for carboxylation of the N7 atom of the BioU conjugate with 8-amino-7-oxononanoate | Synechocystis sp. |
K124A | mutant is not able to form a covalent linkage with 8-amino-7-oxononanoate | Synechocystis sp. |
K124R | mutant is not able to form a covalent linkage with 8-amino-7-oxononanoate | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(8S)-8-amino-7-oxononanoate + [protein]-L-lysine + CO2 | Synechocystis sp. | - |
(7R,8S)-8-amino-7-(carboxyamino)nonanoate + [protein]-(S)-2-amino-6-oxohexanoate | - |
? | |
(8S)-8-amino-7-oxononanoate + [protein]-L-lysine + NAD(P)H + H+ | Synechocystis sp. | - |
[protein]-N6-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysine + H2O + NAD(P)+ | - |
? | |
[protein]-N6-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysine + CO2 + H2O + NAD(P)+ | Synechocystis sp. | - |
(7R,8S)-8-amino-7-(carboxyamino)nonanoate + [protein]-(S)-2-amino-6-oxohexanoate + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | Q55650 | PCC 6803 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(8S)-8-amino-7-oxononanoate + [protein]-L-lysine + CO2 | - |
Synechocystis sp. | (7R,8S)-8-amino-7-(carboxyamino)nonanoate + [protein]-(S)-2-amino-6-oxohexanoate | - |
? | |
(8S)-8-amino-7-oxononanoate + [protein]-L-lysine + NAD(P)H + H+ | - |
Synechocystis sp. | [protein]-N6-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysine + H2O + NAD(P)+ | - |
? | |
[protein]-N6-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysine + CO2 + H2O + NAD(P)+ | - |
Synechocystis sp. | (7R,8S)-8-amino-7-(carboxyamino)nonanoate + [protein]-(S)-2-amino-6-oxohexanoate + NAD(P)H + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37805, mass spectrometry | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
(S)-8-amino-7-oxononanoate synthase | - |
Synechocystis sp. |
bioU | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Synechocystis sp. | |
NADPH | - |
Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is involved in biotin biosynthesis. BioU catalyzes three reactions: formation of covalent linkage with 8-amino-7-oxononanoate to yield a BioU-7,8-diaminononanoate conjugate at the epsilon-amino group of Lys124 of BioU using NAD(P)H, carboxylation of the conjugate to form BioU-7,8-diaminononanoate-carbamic acid, and release of 7,8-diaminononanoate-carbamic acid using NAD(P)+. In this biosynthetic pathway, BioU is a suicide enzyme that loses the Lys124 amino group after a single round of reaction | Synechocystis sp. |