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Literature summary for 2.6.1.117 extracted from

  • Ellens, K.; Richardson, L.; Frelin, O.; Collins, J.; Ribeiro, C.; Hsieh, Y.; Mullen, R.; Hanson, A.
    Evidence that glutamine transaminase and omega-amidase potentially act in tandem to close the methionine salvage cycle in bacteria and plants (2015), Phytochemistry, 113, 160-169 .
    View publication on PubMed
Search for more literature for 2.6.1.117

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Solanum lycopersicum
expression in Escherichia coli Bacillus subtilis
expression in Escherichia coli Zea mays

Inhibitors

Inhibitors Comment Organism Structure
4-(methylsulfanyl)-2-oxobutanoate
-
 Bacillus subtilis
4-(methylsulfanyl)-2-oxobutanoate
-
 Solanum lycopersicum
4-(methylsulfanyl)-2-oxobutanoate
-
 Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Zea mays
0.22
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Solanum lycopersicum
1.91
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 37°C Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Zea mays 5739
-
plastid
-
 Zea mays 9536
-

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O31665
-
-
Bacillus subtilis 168 O31665
-
-
Solanum lycopersicum
-
-
-
Zea mays A0A1D6I5Q5 cf. EC 2.6.1.1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Solanum lycopersicum 2-oxoglutaramate + L-methionine
-
 ?
L-glutamine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Bacillus subtilis 2-oxoglutaramate + L-methionine
-
 ?
L-glutamine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Zea mays 2-oxoglutaramate + L-methionine
-
 ?
L-glutamine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Bacillus subtilis 168 2-oxoglutaramate + L-methionine
-
 ?
L-histidine + 4-(methylsulfanyl)-2-oxobutanoate
-
 Solanum lycopersicum 3-((1H-imidazo)-4-yl)-2-oxopropanoate + L-methionine
-
 ?
additional information enzyme is almost completely specific for glutamine as amino donor, other amino acids giving no more than 1% of the activity measured with glutamine Solanum lycopersicum ?
-
 ?

Synonyms

Synonyms Comment Organism
101251438
-
 Solanum lycopersicum
Solyc11g013170 locus name Solanum lycopersicum
ZEAMMB73_Zm00001d020696
-
 Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Zea mays
0.15
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Solanum lycopersicum
2.32
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 37°C Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.38
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Solanum lycopersicum
1.61
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Zea mays
3.73
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 37°C Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.71
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Solanum lycopersicum
1.26
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 37°C Bacillus subtilis
1.73
-
 4-(methylsulfanyl)-2-oxobutanoate pH 7.5, 25°C Zea mays