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Literature summary for 2.6.1.116 extracted from

  • Palacio, C.M.; Rozeboom, H.J.; Lanfranchi, E.; Meng, Q.; Otzen, M.; Janssen, D.B.
    Biochemical properties of a Pseudomonas aminotransferase involved in caprolactam metabolism (2019), FEBS J., 286, 4086-4102 .
    View publication on PubMed
Search for more literature for 2.6.1.116

Inhibitors

Inhibitors Comment Organism Structure
6-oxohexanoate product inhibition Chromobacterium violaceum
6-oxohexanoate product inhibition Pseudomonas jessenii
6-oxohexanoate product inhibition Vibrio fluvialis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 6-oxohexanoate pH 8.0, 30°C Pseudomonas jessenii
0.56
-
 4-phenylbutylamine pH 8.0, 30°C Chromobacterium violaceum
0.61
-
 benzylamine pH 8.0, 30°C Vibrio fluvialis
0.71
-
 4-phenylbutylamine pH 8.0, 30°C Vibrio fluvialis
0.77
-
 3-Phenylpropylamine pH 8.0, 30°C Vibrio fluvialis
0.9
-
 5-Aminopentanoate pH 8.0, 30°C Pseudomonas jessenii
1
-
 benzylamine pH 8.0, 30°C Chromobacterium violaceum
1.1
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Vibrio fluvialis
1.3
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Chromobacterium violaceum
1.5
-
 4-phenylbutylamine pH 8.0, 30°C Pseudomonas jessenii
1.54
-
 6-aminohexanoate pH 8.0, 30°C Pseudomonas jessenii
1.7
-
 3-Phenylpropylamine pH 8.0, 30°C Chromobacterium violaceum
1.8
-
 benzylamine pH 8.0, 30°C Pseudomonas jessenii
2
-
 6-oxohexanoate pH 8.0, 30°C Vibrio fluvialis
2.1
-
 4-aminobutanoate pH 8.0, 30°C Pseudomonas jessenii
2.1
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Pseudomonas jessenii
2.3
-
 4-aminobutanoate pH 8.0, 30°C Chromobacterium violaceum
2.4
-
 3-Phenylpropylamine pH 8.0, 30°C Pseudomonas jessenii
2.8
-
 6-oxohexanoate pH 8.0, 30°C Chromobacterium violaceum
3.6
-
 2-Phenylethylamine pH 8.0, 30°C Vibrio fluvialis
5
-
 2-Phenylethylamine pH 8.0, 30°C Chromobacterium violaceum
5.7
-
 1-aminopentane pH 8.0, 30°C Chromobacterium violaceum
7.2
-
 1-aminopentane pH 8.0, 30°C Vibrio fluvialis
8.2
-
 5-Aminopentanoate pH 8.0, 30°C Vibrio fluvialis
8.5
-
 1-aminopentane pH 8.0, 30°C Pseudomonas jessenii
8.9
-
 4-aminobutanoate pH 8.0, 30°C Vibrio fluvialis
9.8
-
 5-Aminopentanoate pH 8.0, 30°C Chromobacterium violaceum
10.6
-
 6-aminohexanoate pH 8.0, 30°C Chromobacterium violaceum
71
-
 6-aminohexanoate pH 8.0, 30°C Vibrio fluvialis
81
-
 2-Phenylethylamine pH 8.0, 30°C Pseudomonas jessenii

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum
-
-
-
Pseudomonas jessenii
-
-
-
Vibrio fluvialis F2XBU9 cf. EC 2.6.1.2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9
-
 substrates pyruvate, 6-aminohexanoate, pH 8.0, 30°C Pseudomonas jessenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-phenylethan-1-amine + pyruvate 17% of the activity with 6-aminohexanoate Pseudomonas jessenii 1-phenylethan-1-one + L-alanine
-
 r
(S)-1-phenylethan-1-amine + pyruvate
-
 Chromobacterium violaceum 1-phenylethan-1-one + L-alanine
-
 r
(S)-1-phenylethan-1-amine + pyruvate
-
 Vibrio fluvialis 1-phenylethan-1-one + L-alanine
-
 r
(S)-1-phenylethan-1-amine + pyruvate 100% of the activity with 6-aminohexanoate Pseudomonas jessenii 1-phenylethan-1-one + L-alanine
-
 r
1-aminopentane + pyruvate
-
 Chromobacterium violaceum pentanal + L-alanine
-
 r
1-aminopentane + pyruvate
-
 Vibrio fluvialis pentanal + L-alanine
-
 r
1-aminopentane + pyruvate
-
 Pseudomonas jessenii pentanal + L-alanine
-
 r
2-phenylethylamine + pyruvate
-
 Chromobacterium violaceum 2-phenylethanal + L-alanine
-
 r
2-phenylethylamine + pyruvate
-
 Vibrio fluvialis 2-phenylethanal + L-alanine
-
 r
2-phenylethylamine + pyruvate
-
 Pseudomonas jessenii 2-phenylethanal + L-alanine
-
 r
3-fluorobenzylamine + pyruvate
-
 Chromobacterium violaceum 3-fluorobenzaldehyde + L-alanine
-
 r
3-fluorobenzylamine + pyruvate
-
 Vibrio fluvialis 3-fluorobenzaldehyde + L-alanine
-
 r
3-fluorobenzylamine + pyruvate
-
 Pseudomonas jessenii 3-fluorobenzaldehyde + L-alanine
-
 r
3-phenylpropylamine + pyruvate
-
 Chromobacterium violaceum 3-phenylpropanal + L-alanine
-
 r
3-phenylpropylamine + pyruvate
-
 Vibrio fluvialis 3-phenylpropanal + L-alanine
-
 r
3-phenylpropylamine + pyruvate
-
 Pseudomonas jessenii 3-phenylpropanal + L-alanine
-
 r
4-aminobutanoate + pyruvate
-
 Chromobacterium violaceum 4-oxobutanoate + L-alanine
-
 r
4-aminobutanoate + pyruvate
-
 Vibrio fluvialis 4-oxobutanoate + L-alanine
-
 r
4-aminobutanoate + pyruvate
-
 Pseudomonas jessenii 4-oxobutanoate + L-alanine
-
 r
4-phenylbutylamine + pyruvate
-
 Chromobacterium violaceum 4-phenylbutanal + L-alanine
-
 r
4-phenylbutylamine + pyruvate
-
 Vibrio fluvialis 4-phenylbutanal + L-alanine
-
 r
4-phenylbutylamine + pyruvate
-
 Pseudomonas jessenii 4-phenylbutanal + L-alanine
-
 r
5-aminopentanoate + pyruvate
-
 Chromobacterium violaceum 5-oxopentanoate + L-alanine
-
 r
5-aminopentanoate + pyruvate
-
 Vibrio fluvialis 5-oxopentanoate + L-alanine
-
 r
5-aminopentanoate + pyruvate
-
 Pseudomonas jessenii 5-oxopentanoate + L-alanine
-
 r
6-aminohexanoate + pyruvate
-
 Pseudomonas jessenii 6-oxohexanoate + L-alanine
-
 r
6-aminohexanoate + pyruvate 135% of the activity with (S)-phenylethan-1-amine Vibrio fluvialis 6-oxohexanoate + L-alanine
-
 r
6-aminohexanoate + pyruvate 180% of the activity with (S)-phenylethan-1-amine Chromobacterium violaceum 6-oxohexanoate + L-alanine
-
 r
6-oxohexanoate + L-alanine
-
 Chromobacterium violaceum 6-aminohexanoate + pyruvate
-
 r
6-oxohexanoate + L-alanine
-
 Vibrio fluvialis 6-aminohexanoate + pyruvate
-
 r
6-oxohexanoate + L-alanine
-
 Pseudomonas jessenii 6-aminohexanoate + pyruvate
-
 r
benzylamine + pyruvate
-
 Chromobacterium violaceum benzaldehyde + L-alanine
-
 r
benzylamine + pyruvate
-
 Vibrio fluvialis benzaldehyde + L-alanine
-
 r
benzylamine + pyruvate
-
 Pseudomonas jessenii benzaldehyde + L-alanine
-
 r

Synonyms

Synonyms Comment Organism
CMK94_18730
-
 Pseudomonas jessenii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 5-Aminopentanoate pH 8.0, 30°C Vibrio fluvialis
0.1
-
 4-phenylbutylamine pH 8.0, 30°C Vibrio fluvialis
0.13
-
 3-Phenylpropylamine pH 8.0, 30°C Pseudomonas jessenii
0.18
-
 1-aminopentane pH 8.0, 30°C Pseudomonas jessenii
0.21
-
 5-Aminopentanoate pH 8.0, 30°C Pseudomonas jessenii
0.21
-
 3-Phenylpropylamine pH 8.0, 30°C Vibrio fluvialis
0.26
-
 4-aminobutanoate pH 8.0, 30°C Pseudomonas jessenii
0.3
-
 4-aminobutanoate pH 8.0, 30°C Vibrio fluvialis
0.3
-
 2-Phenylethylamine pH 8.0, 30°C Vibrio fluvialis
0.3
-
 6-aminohexanoate pH 8.0, 30°C Pseudomonas jessenii
0.3
-
 4-phenylbutylamine pH 8.0, 30°C Pseudomonas jessenii
0.35
-
 5-Aminopentanoate pH 8.0, 30°C Chromobacterium violaceum
0.38
-
 3-Phenylpropylamine pH 8.0, 30°C Chromobacterium violaceum
0.43
-
 4-phenylbutylamine pH 8.0, 30°C Chromobacterium violaceum
0.55
-
 4-aminobutanoate pH 8.0, 30°C Chromobacterium violaceum
0.58
-
 6-aminohexanoate pH 8.0, 30°C Vibrio fluvialis
0.6
-
 1-aminopentane pH 8.0, 30°C Vibrio fluvialis
0.72
-
 2-Phenylethylamine pH 8.0, 30°C Chromobacterium violaceum
0.74
-
 benzylamine pH 8.0, 30°C Vibrio fluvialis
0.75
-
 benzylamine pH 8.0, 30°C Pseudomonas jessenii
0.75
-
 2-Phenylethylamine pH 8.0, 30°C Pseudomonas jessenii
0.95
-
 6-aminohexanoate pH 8.0, 30°C Chromobacterium violaceum
1.1
-
 1-aminopentane pH 8.0, 30°C Chromobacterium violaceum
1.1
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Pseudomonas jessenii
1.6
-
 benzylamine pH 8.0, 30°C Chromobacterium violaceum
1.6
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Vibrio fluvialis
1.8
-
 6-oxohexanoate pH 8.0, 30°C Vibrio fluvialis
2.3
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Chromobacterium violaceum
5.4
-
 6-oxohexanoate pH 8.0, 30°C Pseudomonas jessenii
11
-
 6-oxohexanoate pH 8.0, 30°C Chromobacterium violaceum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate fold type I pyridoxal 5'-phosphate enzyme Chromobacterium violaceum
pyridoxal 5'-phosphate fold type I pyridoxal 5'-phosphate enzyme Vibrio fluvialis
pyridoxal 5'-phosphate fold type I pyridoxal 5'-phosphate enzyme Pseudomonas jessenii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.3
-
 6-oxohexanoate pH 8.0, 30°C Chromobacterium violaceum
3.3
-
 6-oxohexanoate pH 8.0, 30°C Pseudomonas jessenii
11
-
 6-oxohexanoate pH 8.0, 30°C Vibrio fluvialis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.003
-
 5-Aminopentanoate pH 8.0, 30°C Vibrio fluvialis
0.008
-
 6-aminohexanoate pH 8.0, 30°C Vibrio fluvialis
0.009
-
 2-Phenylethylamine pH 8.0, 30°C Pseudomonas jessenii
0.02
-
 1-aminopentane pH 8.0, 30°C Pseudomonas jessenii
0.03
-
 4-aminobutanoate pH 8.0, 30°C Vibrio fluvialis
0.04
-
 5-Aminopentanoate pH 8.0, 30°C Chromobacterium violaceum
0.05
-
 3-Phenylpropylamine pH 8.0, 30°C Pseudomonas jessenii
0.08
-
 2-Phenylethylamine pH 8.0, 30°C Vibrio fluvialis
0.08
-
 1-aminopentane pH 8.0, 30°C Vibrio fluvialis
0.09
-
 6-aminohexanoate pH 8.0, 30°C Chromobacterium violaceum
0.1
-
 4-aminobutanoate pH 8.0, 30°C Pseudomonas jessenii
0.1
-
 4-phenylbutylamine pH 8.0, 30°C Vibrio fluvialis
0.2
-
 4-aminobutanoate pH 8.0, 30°C Chromobacterium violaceum
0.2
-
 2-Phenylethylamine pH 8.0, 30°C Chromobacterium violaceum
0.2
-
 5-Aminopentanoate pH 8.0, 30°C Pseudomonas jessenii
0.2
-
 6-aminohexanoate pH 8.0, 30°C Pseudomonas jessenii
0.2
-
 1-aminopentane pH 8.0, 30°C Chromobacterium violaceum
0.2
-
 3-Phenylpropylamine pH 8.0, 30°C Chromobacterium violaceum
0.2
-
 4-phenylbutylamine pH 8.0, 30°C Pseudomonas jessenii
0.3
-
 3-Phenylpropylamine pH 8.0, 30°C Vibrio fluvialis
0.4
-
 benzylamine pH 8.0, 30°C Pseudomonas jessenii
0.5
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Pseudomonas jessenii
0.8
-
 4-phenylbutylamine pH 8.0, 30°C Chromobacterium violaceum
0.9
-
 6-oxohexanoate pH 8.0, 30°C Vibrio fluvialis
1.2
-
 benzylamine pH 8.0, 30°C Vibrio fluvialis
1.5
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Vibrio fluvialis
1.6
-
 benzylamine pH 8.0, 30°C Chromobacterium violaceum
1.7
-
 (S)-1-phenylethan-1-amine pH 8.0, 30°C Chromobacterium violaceum
3.8
-
 6-oxohexanoate pH 8.0, 30°C Chromobacterium violaceum
89
-
 6-oxohexanoate pH 8.0, 30°C Pseudomonas jessenii