BRENDA - Enzyme Database
show all sequences of 2.6.1.11

Mutation of the Erwinia amylovora argD gene causes arginine auxotrophy, nonpathogenicity in apples, and reduced virulence in pears

Ramos, L.S.; Lehman, B.L.; Peter, K.A.; McNellis, T.W.; Appl. Environ. Microbiol. 80, 6739-6749 (2014)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
the pCR2.1-argD complementation plasmid is stably maintained in the argD(1000)::Tn5 transposon mutant growing in host tissues without any antibiotic selection. The pCR2.1-argD complementation plasmid can be useful for the expression of genes, markers, and reporters in Erwinia amylovora growing in planta, without concern about losing the plasmid over time
Erwinia amylovora
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Erwinia amylovora
-
-
-
Erwinia amylovora HKN06P1
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-
-
Application (protein specific)
Application
Commentary
Organism
biotechnology
the pCR2.1-argD complementation plasmid is stably maintained in the argD(1000)::Tn5 transposon mutant growing in host tissues without any antibiotic selection. The pCR2.1-argD complementation plasmid can be useful for the expression of genes, markers, and reporters in Erwinia amylovora growing in planta, without concern about losing the plasmid over time
Erwinia amylovora
General Information
General Information
Commentary
Organism
physiological function
the argD gene encodes a predicted N-acetylornithine aminotransferase enzyme. A mutant having the Tn5 transposon inserted after nucleotide 999 in the argD gene-coding region, is an arginine auxotroph that does not cause fire blight in apple and has reduced virulence in immature pear fruits. Even when mixed with virulent cells and inoculated onto immature apple fruit, the Tn5 mutant still fails to grow. The ArgD protein cannot be considered an Erwinia amylovora virulence factor because the argD(1000)::Tn5 mutant is auxotrophic and has a primary metabolism defect
Erwinia amylovora
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the argD gene encodes a predicted N-acetylornithine aminotransferase enzyme. A mutant having the Tn5 transposon inserted after nucleotide 999 in the argD gene-coding region, is an arginine auxotroph that does not cause fire blight in apple and has reduced virulence in immature pear fruits. Even when mixed with virulent cells and inoculated onto immature apple fruit, the Tn5 mutant still fails to grow. The ArgD protein cannot be considered an Erwinia amylovora virulence factor because the argD(1000)::Tn5 mutant is auxotrophic and has a primary metabolism defect
Erwinia amylovora
Other publictions for EC 2.6.1.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737502
Ramos
Mutation of the Erwinia amylov ...
Erwinia amylovora, Erwinia amylovora HKN06P1
Appl. Environ. Microbiol.
80
6739-6749
2014
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739103
Lal
The redundant aminotransferase ...
Escherichia coli
Mol. Microbiol.
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843-856
2014
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689939
Rajaram
Structure of biosynthetic N-ac ...
Salmonella enterica subsp. enterica serovar Typhimurium
Proteins
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429-441
2008
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671121
Rajaram
Cloning, purification, crystal ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
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980-983
2006
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Vander Wauven
Occurrence of succinyl derivat ...
Burkholderia cepacia
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1985
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Isolation and characterization ...
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Friedrich
Catabolic N2-acetylornithine 5 ...
Klebsiella aerogenes
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Ornithine delta-transaminase a ...
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