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Literature summary for 2.5.1.B45 extracted from
- Sequential regiospecific gem-diprenylation of tetrahydroxyxanthone by prenyltransferases from Hypericum sp. (2019), New Phytol., 222, 318-334 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Nicotiana benthamiana | Hypericum calycinum |
| expressed in Saccharomyces cerevisiae strain InvSc1 | Hypericum sampsonii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0158 | - |
dimethylallyl diphosphate | isoform PT8px, at pH 7.0 and 37°C | Hypericum sampsonii |  |
| 0.0237 | - |
dimethylallyl diphosphate | isoform PTpat, at pH 7.5 and 37°C | Hypericum sampsonii | |
| 0.0532 | - |
1,3,6,7-tetrahydroxyxanthone | isoform PT8px, at pH 7.0 and 37°C | Hypericum sampsonii | |
| 0.0736 | - |
1,3,6,7-tetrahydroxy-8-prenylxanthone | isoform PTpat, at pH 7.5 and 37°C | Hypericum sampsonii | |
| 0.0838 | - |
1,3,6,7-tetrahydroxy-8-prenylxanthone | isoform PT8px, at pH 7.0 and 37°C | Hypericum sampsonii | |
| 0.2 | - |
1,3,6,7-tetrahydroxyxanthone | isoform PTpat, at pH 7.5 and 37°C | Hypericum sampsonii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast envelope | - |
Hypericum calycinum | 9941 | - |
| chloroplast envelope | - |
Hypericum sampsonii | 9941 | - |
| membrane | - |
Hypericum calycinum | 16020 | - |
| membrane | - |
Hypericum sampsonii | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 6 mM used in assay conditions | Hypericum calycinum | |
| Mg2+ | 6 mM used in assay conditions | Hypericum sampsonii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hypericum calycinum | A0A3Q8S421 | - |
- |
| Hypericum calycinum | A0A3S8RK07 | - |
- |
| Hypericum sampsonii | A0A3S5HJL9 | - |
- |
| Hypericum sampsonii | A0A451ENJ9 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Hypericum calycinum | - |
| seedling | - |
Hypericum sampsonii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone | - |
Hypericum calycinum | diphosphate + patulone | - |
? | |
| dimethylallyl diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone | 100% activity with isoform PTpat | Hypericum sampsonii | diphosphate + patulone | - |
? | |
| dimethylallyl diphosphate + 1,3,6,7-tetrahydroxyxanthone | - |
Hypericum calycinum | diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone | - |
? | |
| dimethylallyl diphosphate + 1,3,6,7-tetrahydroxyxanthone | 100% activity with isoform PT8px | Hypericum sampsonii | diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone | - |
? | |
| dimethylallyl diphosphate + 1,3,6,7-tetrahydroxyxanthone | 4.6% activity with isoform PTpat | Hypericum sampsonii | diphosphate + 1,3,6,7-tetrahydroxy-8-prenylxanthone | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| apT | - |
Hypericum calycinum |
| apT | - |
Hypericum sampsonii |
| aromatic prenyltransferase | - |
Hypericum calycinum |
| aromatic prenyltransferase | - |
Hypericum sampsonii |
| PT8px | isoform | Hypericum sampsonii |
| PT8px | isoform | Hypericum calycinum |
| PTpat | isoform | Hypericum calycinum |
| PTpat | isoform | Hypericum sampsonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Hypericum calycinum |
| 35 | 40 | - |
Hypericum sampsonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Hypericum calycinum |
| 7 | 7.5 | - |
Hypericum sampsonii |
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