Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Salinispora tropica CNB-440 |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 28% (w/v) PEG 3350, 0.3 M KCl, 2 mM dithiothreitol, 0.1 M MOPSO, pH 7.0 | Salinispora tropica CNB-440 |
Protein Variants | Comment | Organism |
---|---|---|
G131S | the mutation leads to the complete loss of all SalL halogenase activities and the inability to crystallize SalL | Salinispora tropica CNB-440 |
W129F | the mutant shows 52% chlorinase activity, 76% brominase activity, and 3% iodidase activity compared to the wild type enzyme | Salinispora tropica CNB-440 |
Y70T | the mutation results in a two-orders-of-magnitude reduction of SalL activity, the mutant shows 0.07% chlorinase activity, 0.1% brominase activity, and 0.4% iodidase activity compared to the wild type enzyme | Salinispora tropica CNB-440 |
Y70T/G131S | the mutant shows 0.3% chlorinase activity, 0.5% brominase activity, and no iodidase activity compared to the wild type enzyme | Salinispora tropica CNB-440 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
5'-chloro-5'-deoxyadenosine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.001 | - |
S-adenosyl-L-methionine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.0046 | - |
5'-deoxy-5'-iodoadenosine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
45 | - |
chloride | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
150 | - |
bromide | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
260 | - |
Iodide | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
3 * 30000, in solution as determined by sedimentation velocity studies | Salinispora tropica CNB-440 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salinispora tropica CNB-440 | A4X3Q0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Salinispora tropica CNB-440 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-deoxy-5'-chloroadenosine + L-methionine | - |
Salinispora tropica CNB-440 | S-adenosyl-L-methionine + chloride | - |
r | |
additional information | the enzyme does not accept fluoride and 5-deoxy-5'-fluoroadenosine as substrates | Salinispora tropica CNB-440 | ? | - |
? | |
S-adenosyl-L-methionine + bromide | the enzyme also accepts bromide as substrate (with 90% relative activity compared to chloride) but the brominase activity is probably not biologically relevant in this marine bacterium | Salinispora tropica CNB-440 | 5'-deoxy-5'-bromoadenosine + L-methionine | - |
r | |
S-adenosyl-L-methionine + chloride | - |
Salinispora tropica CNB-440 | 5'-deoxy-5'-chloroadenosine + L-methionine | - |
r | |
S-adenosyl-L-methionine + iodide | the enzyme also accepts iodide as substrate (with 33% relative activity compared to chloride) but the iodinase activity is probably not biologically relevant in this marine bacterium | Salinispora tropica CNB-440 | 5'-deoxy-5'-iodoadenosine + L-methionine | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 30000, in solution as determined by sedimentation velocity studies | Salinispora tropica CNB-440 |
Synonyms | Comment | Organism |
---|---|---|
chlorinase | - |
Salinispora tropica CNB-440 |
SalL | - |
Salinispora tropica CNB-440 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
Iodide | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.013 | - |
bromide | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.015 | - |
S-adenosyl-L-methionine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.015 | - |
chloride | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.2 | - |
L-methionine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.2 | - |
5'-chloro-5'-deoxyadenosine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.83 | - |
5'-deoxy-5'-iodoadenosine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 |
General Information | Comment | Organism |
---|---|---|
physiological function | SalL functions in an orthogonal manner to biological chlorination reactions to initiate the biosynthesis of a new halogenated polyketide synthase building block | Salinispora tropica CNB-440 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000192 | - |
Iodide | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.0000883 | - |
bromide | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.0003 | - |
chloride | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
0.077 | - |
L-methionine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
15 | - |
S-adenosyl-L-methionine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
181.1 | - |
5'-deoxy-5'-iodoadenosine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 | |
1000 | - |
5'-chloro-5'-deoxyadenosine | in 50 mM phosphate buffer (pH 7.9) at 37°C | Salinispora tropica CNB-440 |