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Literature summary for 2.5.1.9 extracted from

  • Fischer, M.; Haase, I.; Feicht, R.; Schramek, N.; Kohler, P.; Schieberle, P.; Bacher, A.
    Evolution of vitamin B2 biosynthesis: riboflavin synthase of Arabidopsis thaliana and its inhibition by riboflavin (2005), Biol. Chem., 386, 417-428.
    View publication on PubMed
Search for more literature for 2.5.1.9

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis, highly effective expression in Escherichia coli by construction of a synthetic gene optimized for heterologous expression by exchange of 59 codons Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
riboflavin sigmoidal product inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, numerical simulation, recombinant enzyme Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21171
-
 3 * 21171, recombinant enzyme expressed from synthetic gene, electrospray mass spectrometry Arabidopsis thaliana
67500
-
 recombinant enzyme expressed from synthetic gene, sedimentation equilibrium centrifugation and gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine Arabidopsis thaliana
-
 riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
additional information Arabidopsis thaliana vitamin B2 biosynthetic pathway evolution, hypothetical gene duplication conductive to the 2-domain architecture occurring very early in evolution ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 AY604007, GenBank synthetic gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-(1-D-ribityl)lumazine
-
 Arabidopsis thaliana riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
additional information vitamin B2 biosynthetic pathway evolution, hypothetical gene duplication conductive to the 2-domain architecture occurring very early in evolution Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 21171, recombinant enzyme expressed from synthetic gene, electrospray mass spectrometry Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.027
-
 6,7-dimethyl-8-(1-D-ribityl)lumazine 25°C, recombinant enzyme Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics, recombinant enzyme Arabidopsis thaliana