Cloned (Comment) | Organism |
---|---|
expression of TcSPPS is able to complement an Escherichia coli ispB mutant | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00459 | - |
farnesyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
0.00707 | - |
geranylgeranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
0.0192 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranyl diphosphate | Trypanosoma cruzi | |
0.0243 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate | Trypanosoma cruzi | |
0.0307 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: farnesyl diphosphate | Trypanosoma cruzi | |
0.0548 | - |
geranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma cruzi | 20015 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal level of activity obtained at 0.5-1 mM. No activation by Mn2+ | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, calculated from sequence | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + 5 isopentenyl diphosphate | Trypanosoma cruzi | the enzyme is involved in synthesis of ubiquinone-9 | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q964Q8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | - |
Trypanosoma cruzi | 6 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
dimethylallyl diphosphate + 8 isopentenyl diphosphate | low activity | Trypanosoma cruzi | 8 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
geranyl diphosphate + 7 isopentenyl diphosphate | - |
Trypanosoma cruzi | 7 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 5 isopentenyl diphosphate | geranylgeranyl diphosphate is the preferred allylic substrate | Trypanosoma cruzi | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
geranylgeranyl diphosphate + 5 isopentenyl diphosphate | the enzyme is involved in synthesis of ubiquinone-9 | Trypanosoma cruzi | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, calculated from sequence | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
solanesyl-diphosphate synthase | - |
Trypanosoma cruzi |
SPPS | - |
Trypanosoma cruzi |
TCSPPS | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
0.043 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: farnesyl diphosphate | Trypanosoma cruzi | |
0.065 | - |
geranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
0.087 | - |
geranylgeranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
0.131 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranyl diphosphate | Trypanosoma cruzi | |
0.217 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate | Trypanosoma cruzi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trypanosoma cruzi | calculated from sequence | - |
6.01 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00119 | - |
geranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
0.0014 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: farnesyl diphosphate | Trypanosoma cruzi | |
0.00685 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranyl diphosphate | Trypanosoma cruzi | |
0.00701 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
0.00893 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate | Trypanosoma cruzi | |
0.012 | - |
geranylgeranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi |