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Literature summary for 2.5.1.7 extracted from

  • Marquardt, J.L.; Siegele, D.A.; Kolter, R.; Walsh, C.T.
    Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase (1992), J. Bacteriol., 174, 5748-5752.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.7

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, chromosome mapping at 69.3 min, overexpression in Escherichia coli strain JLM 16 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
phosphonomycin irreversible inactivation, requires the presence of UDP-GlcNAc Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 phosphoenolpyruvate
-
 Escherichia coli
2.5
-
 UDP-GlcNAc
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44800
-
 MW deduced from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A749 gene murZ, at 69.3 min of the chromosome
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant from Escherichia coli Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Escherichia coli phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.75
-
 phosphoenolpyruvate recombinant enzyme Escherichia coli
4.75
-
 UDP-N-acetyl-D-glucosamine recombinant enzyme Escherichia coli
4.75
-
 phosphoenolpyruvate cosubstrate UDP-N-acetyl-D-glucosamine Escherichia coli
4.75
-
 UDP-N-acetyl-D-glucosamine cosubstrate phosphoenolpyruvate Escherichia coli