Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Lactiplantibacillus paraplantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.47 | - |
L-methionine | at pH 8.5 and 40°C | Lactiplantibacillus paraplantarum | |
2.84 | - |
ATP | at pH 8.5 and 40°C | Lactiplantibacillus paraplantarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Lactiplantibacillus paraplantarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Lactiplantibacillus paraplantarum | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | Lactiplantibacillus paraplantarum 72815 | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus paraplantarum | - |
- |
- |
Lactiplantibacillus paraplantarum 72815 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and gel filtration | Lactiplantibacillus paraplantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Lactiplantibacillus paraplantarum | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | - |
Lactiplantibacillus paraplantarum 72815 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Lactiplantibacillus paraplantarum |
? | x * 42663, calculated from amino acid sequence | Lactiplantibacillus paraplantarum |
Synonyms | Comment | Organism |
---|---|---|
S-adenosyl-L-methionine synthetase | - |
Lactiplantibacillus paraplantarum |
SAM synthetase | - |
Lactiplantibacillus paraplantarum |
SAM-s | - |
Lactiplantibacillus paraplantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lactiplantibacillus paraplantarum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | more than 50% activity between 20 and 50°C. The activity is low below 20°C and above 55°C with less than 20% relative activity | Lactiplantibacillus paraplantarum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
the enzyme is stable with a residual activity over 85% after incubation at 35°C for 2 h | Lactiplantibacillus paraplantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Lactiplantibacillus paraplantarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | more than 60% activity between pH 8.0 and 9.0. There is no activity below pH 6.0 | Lactiplantibacillus paraplantarum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lactiplantibacillus paraplantarum | calculated from amino acid sequence | - |
4.7 |