Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C11N | enzyme retains 10% of the wild-type activity in absence of metal ions. Addition of divalent metal ions does not affect the catalytic activity of the mutant enzyme and the catalytic efficiency, i.e. the ratio of turnover number to Km-value, is reduced only 12fold, the mutant enzyme has become metal-independent | Aquifex pyrophilus |
N26C | activity of the wild-type enzyme is independent of metal ions, the activity of mutant enzyme is decreased by EDTA and increased by Mn2+ and Cd2+ | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibits wild-type enzyme, no inhibition of mutant enzyme C11N | Aquifex pyrophilus | |
EDTA | inhibits wild-type enzyme, no inhibition of mutant enzyme C11N | Aquifex pyrophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
phosphoenolpyruvate | pH 7.5, 37°C, Cd2+ mutant enzyme N26C | Escherichia coli | |
0.006 | - |
phosphoenolpyruvate | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
0.017 | - |
phosphoenolpyruvate | pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated | Aquifex pyrophilus | |
0.019 | - |
phosphoenolpyruvate | pH 7.5, 37°C, Mn2+ mutant enzyme N26C | Escherichia coli | |
0.02 | - |
D-arabinose 5-phosphate | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
0.026 | - |
phosphoenolpyruvate | pH 7.5, 60°C, wild-type Mn2+-enzyme | Aquifex pyrophilus | |
0.032 | - |
phosphoenolpyruvate | pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated | Escherichia coli | |
0.067 | - |
D-arabinose 5-phosphate | pH 7.5, 60°C, wild-type Mn2+-enzyme | Aquifex pyrophilus | |
0.07 | - |
D-arabinose 5-phosphate | pH 7.5, 37°C, Mn2+ mutant enzyme N26C | Escherichia coli | |
0.075 | - |
D-arabinose 5-phosphate | pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated | Escherichia coli | |
0.11 | - |
D-arabinose 5-phosphate | pH 7.5, 37°C, Cd2+ mutant enzyme N26C | Escherichia coli | |
0.14 | - |
D-arabinose 5-phosphate | pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated | Aquifex pyrophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | increase in steady-state rate of wild-type enzyme, Km: 0.0006 mM | Aquifex pyrophilus | |
Iron | wild-type enzyme contains 0.19 mol of iron per mol of enzyme, mutant enzyme c11N contains 0.22 mol of iron per mol of enzyme | Aquifex pyrophilus | |
Magnesium | wild-type enzyme contains 0.05 mol of magnesium per mol of enzyme, mutant enzyme N26C contains 0.4 mol of magnesium per mol of enzyme | Escherichia coli | |
Mn2+ | 200fold increase in steady-state rate of wild-type enzyme, Km: 0.01 mM. Addition of Mn2+ up to 2 mM does not stimulate the reaction rate of C11N mutant | Aquifex pyrophilus | |
Zinc | wild-type enzyme contains 0.05 mol of zinc per mol of enzyme, mutant enzyme N26C contains 0.2 mol of zinc per mol of enzyme | Escherichia coli | |
Zinc | wild-type enzyme contains 0.26 mol per mol of enzyme, mutant enzyme c11N contains 0.02 mol of zinc per mol of enzyme. | Aquifex pyrophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex pyrophilus | Q8GLK7 | - |
- |
Escherichia coli | P0A715 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Escherichia coli | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-arabinose 5-phosphate | - |
Aquifex pyrophilus | 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase | - |
Escherichia coli |
3-deoxy-D-manno-2-octulosonate-8-phosphate synthase | - |
Aquifex pyrophilus |
Kdo8P synthase | - |
Escherichia coli |
Kdo8P synthase | - |
Aquifex pyrophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
phosphoenolpyruvate | pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated | Escherichia coli | |
0.36 | - |
D-arabinose 5-phosphate | pH 7.5, 37°C, mutant enzyme N26C, EDTA-treated | Escherichia coli | |
0.42 | - |
phosphoenolpyruvate | pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated | Aquifex pyrophilus | |
0.42 | - |
D-arabinose 5-phosphate | pH 7.5, 60°C, mutant enzyme C11N, EDTA-treated | Aquifex pyrophilus | |
1.9 | - |
phosphoenolpyruvate | pH 7.5, 37°C, Cd2+ mutant enzyme N26C | Escherichia coli | |
1.9 | - |
D-arabinose 5-phosphate | pH 7.5, 37°C, Cd2+ mutant enzyme N26C | Escherichia coli | |
1.9 | - |
phosphoenolpyruvate | pH 7.5, 37°C, Mn2+ mutant enzyme N26C | Escherichia coli | |
1.9 | - |
D-arabinose 5-phosphate | pH 7.5, 37°C, Mn2+ mutant enzyme N26C | Escherichia coli | |
6.1 | - |
phosphoenolpyruvate | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
6.1 | - |
D-arabinose 5-phosphate | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
9 | - |
phosphoenolpyruvate | pH 7.5, 60°C, wild-type Mn2+-enzyme | Aquifex pyrophilus | |
9 | - |
D-arabinose 5-phosphate | pH 7.5, 60°C, wild-type Mn2+-enzyme | Aquifex pyrophilus |