Cloned (Comment) | Organism |
---|---|
gene aroF, cloning of mutant enzymes in Escherichia coli strain JM109, recombinant overexpression of mutant enzyme variants AroFD(1-9), AroFD(1-10), AroFD(1-12) and AroFD(1-11) | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | random mutagenesis, the AroF variant with a deficiency in residue Ile11 is insensitive to L-tyrosine. Construction of feedback-resistant 3-deoxy-D-arabino-heptulosonate-7-phosphate synthases by engineering the N-terminal domain for L-phenylalanine synthesis, generation of nine AroF variants with truncation of different N-terminal fragments, the variants AroFD(1-9), AroFD(1-10), AroFD(1-12) and, in particular, AroFD(1-11) significantly lead to the accumulation of L-phenylalanine, overview. Mutant AroFDELTA(1-11) does not show any feedback inhibition by L-Phe, demonstrated in a strain that has a high L-Phe level through coexpression of L-Phe producing chorismate mutase-4-prephenate dehydratase | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tyrosine | feedback inhibition of isozyme AroF | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | P35170 | gene aroF | - |
Corynebacterium glutamicum ATCC 13032 | P35170 | gene aroF | - |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-arabino-heptulosonate-7-phosphate synthase | - |
Corynebacterium glutamicum |
aroF | - |
Corynebacterium glutamicum |
DAHP synthase | - |
Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
metabolism | first enzyme of the shikimate pathway | Corynebacterium glutamicum |