Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chorismate | competitive against phosphoenolpyruvate, noncompetitive against D-erythrose 4-phosphate; DAHP synthase-trp | Pseudomonas aeruginosa | |
phenylpyruvate | DAHP synthase-tyr | Pseudomonas aeruginosa | |
Trp | DAHP synthase-trp; non-competitive against phosphoenolpyruvate and competitive for D-erythrose 4-phosphate; Trp-sensitive isozyme | Pseudomonas aeruginosa | |
Tyr | DAHP synthase-tyr; L-Tyr; Tyr-sensitive isozyme | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
D-erythrose 4-phosphate | DAHP synthase-trp, pH 7.0, 37°C | Pseudomonas aeruginosa | |
1 | - |
phosphoenolpyruvate | DAHP synthase-tyr, pH 7.0, 37°C | Pseudomonas aeruginosa | |
1 | - |
D-erythrose 4-phosphate | DAHP synthase-tyr, pH 7.0, 37°C | Pseudomonas aeruginosa | |
1.11 | - |
phosphoenolpyruvate | DAHP synthase-trp, pH 7.0, 37°C | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
137000 | - |
DAHP synthase-tyr, gel filtration | Pseudomonas aeruginosa |
175000 | - |
DAHP synthase-trp, gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
2 isoenzymes: tyrosine-sensitive DAHP synthase-tyr and tryptophane-sensitive DAHP synthase-trp | - |
Purification (Comment) | Organism |
---|---|
partially, 2 isozymes | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
partially purified isozyme synthase-trp | Pseudomonas aeruginosa |
0.056 | - |
partially purified isozyme synthase-tyr | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Pseudomonas aeruginosa | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
DAHP synthase-tyr | Pseudomonas aeruginosa |
7 | - |
assay at | Pseudomonas aeruginosa |
7.2 | - |
DAHP synthase-trp | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
Trp | synthase-trp isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 | Pseudomonas aeruginosa | |
0.023 | - |
Tyr | synthase-tyr isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 | Pseudomonas aeruginosa | |
0.023 | - |
Tyr | synthase-tyr isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 | Pseudomonas aeruginosa | |
0.04 | - |
Trp | synthase-trp isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 | Pseudomonas aeruginosa | |
1.35 | - |
chorismate | synthase-trp isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 | Pseudomonas aeruginosa | |
1.35 | - |
phenylpyruvate | synthase-tyr isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 | Pseudomonas aeruginosa | |
2.25 | - |
chorismate | synthase-trp isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 | Pseudomonas aeruginosa | |
2.55 | - |
phenylpyruvate | synthase-tyr isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 | Pseudomonas aeruginosa |