Application | Comment | Organism |
---|---|---|
synthesis | enzme OASS produces novel beta-substituted L-amino acids when offered unnatural nucleophiles instead of sulfid, which is useful in producing pharmaceuticals, such as mucolytic agent L-carbocisteine and building blocks for the synthesis of pharmaceuticals and agrochemicals | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | Escherichia coli | - |
L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | Escherichia coli W3110 / ATCC 27325 | - |
L-cysteine + acetate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
1,4-dioxane | the activity of enzyme OASS-B gradually decreases as the content of organic solvent increases | Escherichia coli |
N,N-dimethylformamide | the activity of enzyme OASS-B gradually decreases as the content of organic solvent increases | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli W3110 / ATCC 27325 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography and gel filtration | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate | ping-pong bi-bi mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
pH 7.0, 37°C, 100 mM potassium phosphate | Escherichia coli |
667 | - |
pH 7.5, 25°C, 50 mM potassium phosphate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | - |
Escherichia coli | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | residue Arg210 near the entrance of the active site and is important for O-acetyl-L-serine substrate recognition | Escherichia coli | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | - |
Escherichia coli W3110 / ATCC 27325 | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | residue Arg210 near the entrance of the active site and is important for O-acetyl-L-serine substrate recognition | Escherichia coli W3110 / ATCC 27325 | L-cysteine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-acetyl-L-serine sulfhydrylase B | - |
Escherichia coli |
OASS-B | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | purified recombinant His-tagged enzyme, 1 h, completely stable at | Escherichia coli |
57 | 60 | purified recombinant His-tagged enzyme, 1 h, 50% activity remaining | Escherichia coli |
70 | - |
purified recombinant His-tagged enzyme, 1 h, inactivation | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
additional information | intramolecular electrostatic interaction of enzyme OASS-B, overview | Escherichia coli |