Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as GST-fusion proteins | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
Sulfide | - |
Glycine max | |
0.063 | - |
O-acetyl-L-serine | - |
Glycine max | |
0.105 | - |
O-acetyl-L-serine | - |
Glycine max | |
0.11 | - |
Sulfide | - |
Glycine max | |
0.116 | - |
O-acetyl-L-serine | - |
Glycine max | |
0.355 | - |
O-acetyl-L-serine | - |
Glycine max | |
0.53 | - |
Sulfide | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
calculated from cDNA | Glycine max |
60000 | - |
calculated from cDNA | Glycine max |
66000 | - |
calculated from cDNA | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | A3RM03 | - |
- |
Glycine max | A3RM04 | - |
- |
Glycine max | A3RM05 | - |
- |
Glycine max | A3RM06 | - |
- |
Glycine max | A5YT86 | - |
- |
Glycine max | A5YT88 | - |
- |
Purification (Comment) | Organism |
---|---|
using gel filtration | Glycine max |
using Glutathione Sepharose 4B column | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Glycine max | - |
leaf | - |
Glycine max | - |
root | roots of seedlings | Glycine max | - |
seed | - |
Glycine max | - |
seed | mainly expressed in developing seeds | Glycine max | - |
stem | young stems | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.262 | - |
- |
Glycine max |
0.419 | - |
- |
Glycine max |
0.489 | - |
- |
Glycine max |
0.724 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + sulfide | - |
Glycine max | L-Cys + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GmOAS-TL1 | - |
Glycine max |
GmOAS-TL2 | - |
Glycine max |
GmOAS-TL3 | - |
Glycine max |
GmOAS-TL4 | - |
Glycine max |
GmOAS-TL6 | - |
Glycine max |
GmOAS-TL7 | - |
Glycine max |
General Information | Comment | Organism |
---|---|---|
physiological function | cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL1 grow in the M9 minimal medium in the absence of cysteine | Glycine max |
physiological function | cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL2 does not grow in the M9 minimal medium in the absence of cysteine | Glycine max |
physiological function | cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL3 grow in the M9 minimal medium in the absence of cysteine | Glycine max |
physiological function | cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL4 grow in the M9 minimal medium in the absence of cysteine | Glycine max |
physiological function | cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL6 grow in the M9 minimal medium in the absence of cysteine | Glycine max |
physiological function | cysteine auxotrophic mutant Escherichia coli NK3 transformed with GmOAS-TL7 does not grow in the M9 minimal medium in the absence of cysteine | Glycine max |