Protein Variants | Comment | Organism |
---|---|---|
D26A | site-directed mutagenesis | Escherichia coli |
S83A | site-directed mutagenesis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
farnesyl thiodiphosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | fluorescence-based UPPS kinetic measurements, kinetics of the single-turnover reaction of (2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate chain elongation catalyzed by UPPS, overview | Escherichia coli | |
0.0004 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Escherichia coli | |
0.0011 | 0.0015 | (2E,6E)-8-(N-methyl-2-aminobenzoyloxy)-3,7-dimethyl-2,6-octandien-1-diphosphate | pH 7.5, 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | Escherichia coli | - |
8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-8-(N-methyl-2-aminobenzoyloxy)-3,7-dimethyl-2,6-octandien-1-diphosphate + 8 isopentenyl diphosphate | a fluorescent analog of farnesyl diphosphate | Escherichia coli | ? | - |
? | |
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Escherichia coli | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
undecaprenyl pyrophosphate synthase | - |
Escherichia coli |
UPPs | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 25°C | Escherichia coli | |
11.2 | - |
(2E,6E)-8-(N-methyl-2-aminobenzoyloxy)-3,7-dimethyl-2,6-octandien-1-diphosphate | pH 7.5, 25°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0013 | - |
pH 7.5, 25°C, versus (2E,6E)-farnesyl diphosphate | Escherichia coli | farnesyl thiodiphosphate | |
0.0024 | 0.0039 | pH 7.5, 25°C, versus (2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate | Escherichia coli | farnesyl thiodiphosphate |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
isopentenyl diphosphate | pH 7.5, 25°C, with (2E,6E)-farnesyl diphosphate | Escherichia coli | |
0.0232 | 0.0279 | isopentenyl diphosphate | pH 7.5, 25°C, with (2E,6E)-8-O-(N-methyl-2-aminobenzoyl)-3,7-dimethyl-2,6-octandien-1-diphosphate | Escherichia coli |