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Literature summary for 2.5.1.29 extracted from

  • Feng, Y.; Morgan, R.M.L.; Fraser, P.D.; Hellgardt, K.; Nixon, P.J.
    Crystal structure of geranylgeranyl pyrophosphate synthase (CrtE) involved in cyanobacterial terpenoid biosynthesis (2020), Front. Plant Sci., 11, 589 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.29

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) as an N-terminal histidine-tagged ubiquitin fusion protein using the high-level expression vector pHUE Synechococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method at 16°C. The crystal structure of CrtE solved to a resolution of 2.7 A reveals a strong structural similarity to the large subunit of the heterodimeric geranylgeranylpyrophosphate synthase 1 from Arabidopsis thaliana with each subunit containing 14 helices Synechococcus sp.
sitting drop vapor diffusion method at 16°C. The crystal structure of CrtE solves to a resolution of 2.7 A. Strong structural similarity to the large subunit of the heterodimeric geranylgeranyl pyrophosphate synthase 1 from Arabidopsis thaliana with each subunit containing 14 helices Synechococcus sp.

Protein Variants

Protein Variants Comment Organism
M87A mutant enzyme produces the longer C25-geranylfarnesyl pyrophosphate product Synechococcus sp.
M87F the mutant enzyme can only generate C15-farnesyl pyrophosphate Synechococcus sp.
M87F the mutant enzyme can only generate farnesyl diphosphate, indicating that residues with large side chains obstruct product elongation Synechococcus sp.
S88Y the mutant enzyme can only generate C15-farnesyl pyrophosphate Synechococcus sp.
S88Y the mutant enzyme can only generate farnesyl diphosphate, indicating that residues with large side chains obstruct product elongation Synechococcus sp.
V161M the mutant enzyme, similar amounts of farnesyl diphosphate and geranylgeranyl diphosphate are produced and there is no evidence for formation of geranyl diphosphate Synechococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 gel filtration Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Synechococcus sp. the enzyme is involved in Cyanobacterial terpenoid biosynthesis diphosphate + geranylgeranyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate Synechococcus sp. ATCC 27264 the enzyme is involved in Cyanobacterial terpenoid biosynthesis diphosphate + geranylgeranyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Synechococcus sp. B1XJV9
-
-
Synechococcus sp. ATCC 27264 B1XJV9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Synechococcus sp. diphosphate + geranylgeranyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate the enzyme is involved in Cyanobacterial terpenoid biosynthesis Synechococcus sp. diphosphate + geranylgeranyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Synechococcus sp. ATCC 27264 diphosphate + geranylgeranyl diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate the enzyme is involved in Cyanobacterial terpenoid biosynthesis Synechococcus sp. ATCC 27264 diphosphate + geranylgeranyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Synechococcus sp.

Synonyms

Synonyms Comment Organism
CrtE
-
 Synechococcus sp.
geranylgeranyl pyrophosphate synthase
-
 Synechococcus sp.

General Information

General Information Comment Organism
physiological function the enzyme is involved in Cyanobacterial terpenoid biosynthesis Synechococcus sp.