Crystallization (Comment) | Organism |
---|---|
the 3D structure of GGPPS reveals an unique positioning of the N-terminal helix A, which protrudes into the other subunit and stabilizes dimerization, although it is far from the main dimer interface. The replacement of residues L8 and I9 at this helix with Gly is sufficient to disrupt the dimer into a monomer, leading to at least 1000fold reduction in activity | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
DELTA 1-17 | complete loss of activity, monomer | Saccharomyces cerevisiae |
DELTA1-6 | 72% of wild-type activity. Dimer formation | Saccharomyces cerevisiae |
DELTA1-7 | 29% of wild-type activity. Dimer and monomer formation | Saccharomyces cerevisiae |
Delta1-8 | 0.3% of wild-type activity. Monomer formation | Saccharomyces cerevisiae |
DELTA1-9 | 0.2% of wild-type activity. Monomer formation | Saccharomyces cerevisiae |
E7G | 84% of wild-type activity. Dimer and monomer formation | Saccharomyces cerevisiae |
E7G/L8G | 0.6% of wild-type activity | Saccharomyces cerevisiae |
I9G | 2.5% of wild-type activity | Saccharomyces cerevisiae |
L8G | 20% of wild-type activity. Dimer formation | Saccharomyces cerevisiae |
L8G/I9G | 0.3% of wild-type activity. Monomer formation | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
isopentenyl diphosphate | mutant E7G/L8G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0004 | - |
isopentenyl diphosphate | mutant DELTA1-7, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0004 | - |
isopentenyl diphosphate | mutant E7G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0005 | - |
isopentenyl diphosphate | mutant DELTA1-8, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0005 | - |
isopentenyl diphosphate | mutant L8G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0006 | - |
isopentenyl diphosphate | mutant DELTA1-6, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0006 | - |
isopentenyl diphosphate | mutant L8G/I9G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0007 | - |
isopentenyl diphosphate | mutant I9G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0008 | - |
isopentenyl diphosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0014 | - |
(E,E)-farnesyl diphosphate | mutant DELTA1-7, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0014 | - |
isopentenyl diphosphate | mutant DELTA1-9, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0019 | - |
(E,E)-farnesyl diphosphate | mutant DELTA1-9, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0024 | - |
(E,E)-farnesyl diphosphate | mutant L8G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0026 | - |
(E,E)-farnesyl diphosphate | mutant DELTA1-8, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0028 | - |
(E,E)-farnesyl diphosphate | mutant E7G/L8G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0031 | - |
(E,E)-farnesyl diphosphate | mutant L8G/I9G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0032 | - |
(E,E)-farnesyl diphosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0036 | - |
(E,E)-farnesyl diphosphate | mutant I9G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0043 | - |
(E,E)-farnesyl diphosphate | mutant DELTA1-6, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0044 | - |
(E,E)-farnesyl diphosphate | mutant E7G, pH 7.5, 25°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12051 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Saccharomyces cerevisiae | diphosphate + geranylgeranyl diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000042 | - |
(E,E)-farnesyl diphosphate | mutant DELTA1-9, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.000066 | - |
(E,E)-farnesyl diphosphate | mutant DELTA1-8, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.000072 | - |
(E,E)-farnesyl diphosphate | mutant L8G/I9G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.00015 | - |
(E,E)-farnesyl diphosphate | mutant E7G/L8G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.00062 | - |
(E,E)-farnesyl diphosphate | mutant I9G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0051 | - |
(E,E)-farnesyl diphosphate | mutant L8G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.0073 | - |
(E,E)-farnesyl diphosphate | mutant DELTA1-7, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.018 | - |
(E,E)-farnesyl diphosphate | mutant DELTA1-6, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.021 | - |
(E,E)-farnesyl diphosphate | mutant E7G, pH 7.5, 25°C | Saccharomyces cerevisiae | |
0.025 | - |
(E,E)-farnesyl diphosphate | wild-type, pH 7.5, 25°C | Saccharomyces cerevisiae |