Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Taxus canadensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
farnesyl diphosphate | recombinant enzyme | Taxus canadensis | |
0.007 | - |
isopentenyl diphosphate | recombinant enzyme | Taxus canadensis | |
0.035 | - |
Mg2+ | recombinant enzyme | Taxus canadensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Taxus canadensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31500 | - |
2 * 31500, deduced from nucleotide sequence | Taxus canadensis |
60000 | - |
recombinant truncated enzyme, gel filtration | Taxus canadensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | Taxus canadensis | 66% of activity with geranyl diphosphate | geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | Taxus canadensis | 33% of activity with geranyl diphosphate | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Taxus canadensis | preferred substrate | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Taxus canadensis | involved in taxol biosynthesis | geranylgeranyl diphosphate + 2 diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus canadensis | Q9ZPM3 | canadian yew | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Taxus canadensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Taxus canadensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | 66% of activity with geranyl diphosphate | Taxus canadensis | geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | 33% of activity with geranyl diphosphate | Taxus canadensis | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | preferred substrate | Taxus canadensis | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | involved in taxol biosynthesis | Taxus canadensis | geranylgeranyl diphosphate + 2 diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31500, deduced from nucleotide sequence | Taxus canadensis |