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Literature summary for 2.5.1.20 extracted from

  • Kang, H.; Kang, M.Y.; Han, K.H.
    Identification of natural rubber and characterization of rubber biosynthetic activity in fig tree (2000), Plant Physiol., 123, 1133-1142.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
EDTA approx. 3.5fold activation at 20 mM Ficus carica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
farnesyl diphosphate
-
Ficus carica
0.228
-
isopentenyl diphosphate
-
Ficus carica

Localization

Localization Comment Organism GeneOntology No. Textmining
particle-bound
-
Hevea brasiliensis
-
-
particle-bound associated with rubber particles Ficus carica
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Hevea brasiliensis
Mg2+ required for activity Ficus carica
Mg2+ maximal activity at 1 mM, inhibition above 10 mM Ficus carica
Mg2+ maximal activity at 2 mM, inhibition above 10 mM Hevea brasiliensis

Organism

Organism UniProt Comment Textmining
Ficus carica
-
-
-
Hevea brasiliensis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
Hevea brasiliensis
-
latex associated with rubber particles Ficus carica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyl diphosphate + cis-1,4-polyisoprene
-
Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?
isopentenyl diphosphate + cis-1,4-polyisoprene
-
Ficus carica diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?
isopentenyl diphosphate + farnesyl diphosphate + cis-1,4-polyisoprene an allylic diphosphate e.g. farnesyl diphosphate is required to initiate the biosynthesis of new rubber molecules Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?
isopentenyl diphosphate + farnesyl diphosphate + cis-1,4-polyisoprene an allylic diphosphate e.g. farnesyl diphosphate is required to initiate the biosynthesis of new rubber molecules Ficus carica diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?