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Literature summary for 2.5.1.18 extracted from
- Biochemical characterization of an unclassified glutathione S-transferase of Plutella xylostella (2016), J. Pestic. Sci., 41, 145-151 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) pLysS cells | Plutella xylostella |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bendiocarb | - |
Plutella xylostella |  |
| chlorfenapyr | - |
Plutella xylostella | |
| diazinon | about 30% residual activity at 1 mM | Plutella xylostella | |
| imidacloprid | about 30% residual activity at 1 mM | Plutella xylostella | |
| permethrin | about 50% residual activity at 1 mM | Plutella xylostella | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.57 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.0 and 30°C | Plutella xylostella | |
| 2.1 | - |
Ethacrynic acid | at pH 7.0 and 30°C | Plutella xylostella | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plutella xylostella | - |
diamondback moth | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-affinity column chromatography and Superdex S200 gel filtration | Plutella xylostella |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.041 | - |
with 1-chloro-2,4-dinitrobenzene as substrate, at pH 7.0 and 30°C | Plutella xylostella |
| 0.041 | - |
with ethacrynic acid as substrate, at pH 7.0 and 30°C | Plutella xylostella |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Plutella xylostella | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
| glutathione + ethacrynic acid | best substrate | Plutella xylostella | ? | - |
? | |
| additional information | no activity with 1,2-epoxy-3-(4-nitrophenoxy) propane, 4-nitrobenzyl chloride, 4-nitrophenethyl bromide, 4-hydroxynonenal, 4-nitrophenyl acetate, and H2O2 | Plutella xylostella | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE | Plutella xylostella |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Plutella xylostella |
| GSTU1 | - |
Plutella xylostella |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | the enzyme shows about 100%, 80%, 60%, 30% and no activity after 30 min at 30, 40, 50, 60, and 70°C | Plutella xylostella |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Plutella xylostella |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | the enzyme retains more than 80% original activity at pH values of 7.0 to 10.0 | Plutella xylostella |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | the enzyme remains stable for 24 h at pH 7.0-10.0 and retains about 50% and less than 10% activity after 24 h at pH 6.0 and 11.0, respectively | Plutella xylostella |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme contributes to insecticide metabolism in the diamondback moth | Plutella xylostella |
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Release 2023.1 (January 2023)
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