Cloned (Comment) | Organism |
---|---|
gene GSTA1, from ovary and testis, DNA and amino acid sequence determination and analysis, expression of isozyme GST A1-1 in Escherichia coli strain Rosetta(DE3) | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.121 | - |
phenethyl isothiocyanate | pH 7.8, 30°C | Sus scrofa | |
0.16 | - |
trans-2-nonenal | pH 7.8, 30°C | Sus scrofa | |
0.2 | - |
1-chloro-2,4-dinitrobenzene | pH 7.8, 30°C | Sus scrofa | |
0.237 | - |
5-androstene-3,17-dione | pH 7.8, 30°C | Sus scrofa | |
0.7 | - |
5-Pregnene-3,20-dione | pH 7.8, 30°C | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P51781 | gene GSTA1 | - |
Sus scrofa Yorkshire pig | P51781 | gene GSTA1 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme GST A1-1 from Escherichia coli by ammonium sulfate fractionation, gel filtration, S-hexylglutathione affinity chromatography, ultrafiltration, and again gel filtration | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | - |
Sus scrofa | - |
liver | - |
Sus scrofa | - |
additional information | tissue distribution of the GSTA1 mRNA, overview | Sus scrofa | - |
ovary | - |
Sus scrofa | - |
pituitary gland | - |
Sus scrofa | - |
testis | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Sus scrofa | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
1-chloro-2,4-dinitrobenzene + glutathione | - |
Sus scrofa Yorkshire pig | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
5-androstene-3,17-dione + glutathione | - |
Sus scrofa | ? | - |
? | |
5-androstene-3,17-dione + glutathione | - |
Sus scrofa Yorkshire pig | ? | - |
? | |
5-pregnene-3,20-dione + glutathione | - |
Sus scrofa | ? | - |
? | |
5-pregnene-3,20-dione + glutathione | - |
Sus scrofa Yorkshire pig | ? | - |
? | |
additional information | the enzyme is active with cumene hydroperoxide. The GST A1-1 catalytic activity in steroid isomerization reactions is at least 10fold lower than the corresponding values for porcine GST A2-2, whereas the activity with 1-chloro-2,4-dinitrobenzene is approximately 8fold higher. Differences in the H-site residues of mammalian Alpha-class GSTs may explain the catalytic divergence | Sus scrofa | ? | - |
? | |
additional information | the enzyme is active with cumene hydroperoxide. The GST A1-1 catalytic activity in steroid isomerization reactions is at least 10fold lower than the corresponding values for porcine GST A2-2, whereas the activity with 1-chloro-2,4-dinitrobenzene is approximately 8fold higher. Differences in the H-site residues of mammalian Alpha-class GSTs may explain the catalytic divergence | Sus scrofa Yorkshire pig | ? | - |
? | |
phenethyl isothiocyanate + glutathione | - |
Sus scrofa | ? | - |
? | |
phenethyl isothiocyanate + glutathione | - |
Sus scrofa Yorkshire pig | ? | - |
? | |
trans-2-nonenal + glutathione | - |
Sus scrofa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-class glutathione transferase | - |
Sus scrofa |
glutathione transferase A1-1 | - |
Sus scrofa |
GST A1-1 | - |
Sus scrofa |
pGSTA1 | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5-Pregnene-3,20-dione | pH 7.8, 30°C | Sus scrofa | |
0.155 | - |
trans-2-nonenal | pH 7.8, 30°C | Sus scrofa | |
4.8 | - |
5-androstene-3,17-dione | pH 7.8, 30°C | Sus scrofa | |
9.5 | - |
phenethyl isothiocyanate | pH 7.8, 30°C | Sus scrofa | |
84 | - |
1-chloro-2,4-dinitrobenzene | pH 7.8, 30°C | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Sus scrofa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sus scrofa | GST A1-1 | - |
9 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
trans-2-nonenal | pH 7.8, 30°C | Sus scrofa | |
16 | - |
5-Pregnene-3,20-dione | pH 7.8, 30°C | Sus scrofa | |
20 | - |
5-androstene-3,17-dione | pH 7.8, 30°C | Sus scrofa | |
79 | - |
phenethyl isothiocyanate | pH 7.8, 30°C | Sus scrofa | |
420 | - |
1-chloro-2,4-dinitrobenzene | pH 7.8, 30°C | Sus scrofa |