Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
F91W | with cob(II)alamin the mutant shows catalytic activity diminished relative to that of wild type enzyme, while the activity with cob(I)alamin is largely retained | Salmonella enterica |
F91W/W93F | in this mutant the yield of 4c cob(II)alamin species is reduced more than 10fold from that achieved by the wild type enzyme | Salmonella enterica |
F91Y | with cob(II)alamin the mutant shows catalytic activity 5fold increased relative to that of wild type enzyme | Salmonella enterica |
W93A | the mutation completely abolishes the catalytic activity with cob(II)alamin while modest activity is retained with cob(I)alamin | Salmonella enterica |
W93F | the mutant shows wild type activity | Salmonella enterica |
W93H | the mutation inhibits the formation of 4c cob(II)alamin | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0163 | - |
cob(II)inamide | wild type enzyme, pH and temperature not specified in the publication | Salmonella enterica | |
0.0254 | - |
ATP | wild type enzyme, pH and temperature not specified in the publication | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)yrinic acid a,c-diamide | Salmonella enterica | - |
triphosphate + adenosylcob(III)yrinic acid a,c-diamide | - |
? | |
ATP + cobinamide | Salmonella enterica | - |
triphosphate + adenosylcobinamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | P31570 | serovar typhimurium LT2 | - |
Purification (Comment) | Organism |
---|---|
HisTrap nickel affinity column chromatography | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamine | - |
Salmonella enterica | triphosphate + adenosylcob(I)alamine | - |
? | |
ATP + cob(I)yrinic acid a,c-diamide | - |
Salmonella enterica | triphosphate + adenosylcob(III)yrinic acid a,c-diamide | - |
? | |
ATP + cob(II)alamine | - |
Salmonella enterica | triphosphate + adenosylcob(II)alamine | - |
? | |
ATP + cob(II)inamide | - |
Salmonella enterica | triphosphate + adenosylcob(II)inamide | - |
? | |
ATP + cobinamide | - |
Salmonella enterica | triphosphate + adenosylcobinamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACAT | - |
Salmonella enterica |
ATP:co(I)rrinoid adenosyltransferase | - |
Salmonella enterica |
CobA | - |
Salmonella enterica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
ATP | wild type enzyme, pH and temperature not specified in the publication | Salmonella enterica | |
0.0077 | - |
cob(II)inamide | wild type enzyme, pH and temperature not specified in the publication | Salmonella enterica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
ATP | wild type enzyme, pH and temperature not specified in the publication | Salmonella enterica | |
0.47 | - |
cob(II)inamide | wild type enzyme, pH and temperature not specified in the publication | Salmonella enterica |