General Stability | Organism |
---|---|
freeze-thawing inactivates | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-ethylthioadenosine | - |
Escherichia coli | |
additional information | inhibition by end products of reaction; no inhibition by carbonyl binding reagents | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli | |
spermidine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
S-adenosyl-3-methylthio-1-propylamine | pH 8.2, 37ºC, spectrophotometric assay | Escherichia coli | |
0.012 | - |
1,4-diaminobutane | pH 8.2, 37ºC, spectrophotometric assay | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, sedimentation equilibrium centrifugation and SDS-PAGE | Escherichia coli |
72000 | - |
W strain | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Escherichia coli | - |
5'-methylthioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
extraction, ammonium sulfate fractionation, chromatography on calcium phosphate cellulose, DEAE-Sephadex, chromatography on hydroxyapatite, disc gel electrophoresis | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.83 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
0-4°C, purified enzyme, 10-20% loss of activity within 6 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + spermidine | at a reduced rate | Escherichia coli | 5'-methylthioadenosine + spermine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane | - |
Escherichia coli | 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, sedimentation equilibrium centrifugation and SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.4 | - |
- |
Escherichia coli |