Literature summary for 2.5.1.108 extracted from

Fenwick, M.; Dong, M.; Lin, H.; Ealick, S.
The crystal structure of Dph2 in complex with elongation factor 2 reveals the structural basis for the first step of diphthamide biosynthesis (2019), Biochemistry, 58, 4343-4351 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli NiCo21(DE3) cells	Methanobrevibacter smithii

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in complex with elongation factor 2, hanging drop vapor diffusion method, using 100 mM Tris (pH 7.2), 200 mM MgSO4, and 21% (w/v) PEG 400	Methanobrevibacter smithii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2]	Methanobrevibacter smithii	-	S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]	-	?
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2]	Methanobrevibacter smithii DSM 861	-	S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanobrevibacter smithii	A5UMY5	-	-
Methanobrevibacter smithii DSM 861	A5UMY5	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized nickel and benzamidine affinity column chromatography	Methanobrevibacter smithii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2]	-	Methanobrevibacter smithii	S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]	-	?
S-adenosyl-L-methionine + L-histidine-[translation elongation factor 2]	-	Methanobrevibacter smithii DSM 861	S-methyl-5'-thioadenosine + 2-[(3S)-3-amino-3-carboxypropyl]-L-histidine-[translation elongation factor 2]	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 37000, SDS-PAGE	Methanobrevibacter smithii

Synonyms

Synonyms	Comment	Organism
diphthine synthase	-	Methanobrevibacter smithii
Dph2	-	Methanobrevibacter smithii

Cofactor

Cofactor	Comment	Organism	Structure
[4Fe-4S]-center	-	Methanobrevibacter smithii

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Release 2023.1 (January 2023)