Literature summary for 2.5.1.104 extracted from

  • Ohnuma, M.; Terui, Y.; Tamakoshi, M.; Mitome, H.; Niitsu, M.; Samejima, K.; Kawashima, E.; Oshima, T.
    N1-aminopropylagmatine, a new polyamine produced as a key intermediate in polyamine biosynthesis of an extreme thermophile, Thermus thermophilus (2005), J. Biol. Chem., 280, 30073-30082.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.77
-
agmatine pH 9.0, 37°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
x * 32500, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + agmatine Thermus thermophilus the enzyme is involved in polyamine biosynthesis 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SK28
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
pH 8.5, 60°C Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + agmatine
-
Thermus thermophilus 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
?
S-adenosyl 3-(methylthio)propylamine + agmatine the enzyme is involved in polyamine biosynthesis Thermus thermophilus 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
?

Subunits

Subunits Comment Organism
? x * 32500, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
SpeE
-
Thermus thermophilus
agmatine aminopropyltransferase
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
agmatine pH 9.0, 37°C Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Thermus thermophilus

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
480
-
agmatine pH 9.0, 37°C Thermus thermophilus