Crystallization (Comment) | Organism |
---|---|
enzyme in complexes with three different EGF-like domains and either UDP, UDP-2-deoxy-2-fluoro-glucose or UDP-methyl-glucose, hanging drop vapor diffusion method, using 10-20% (w/v) PEG 5000 MME, 50 mM MES pH 6.5, 2-10 mM CaCl2, 0-250 mM NaCl, 5-10% (v/v) glycerol or 2-methyl-2,4-pentanediol | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP-2-deoxy-2-fluoro-glucose | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme does not require a diserine-containing glycosylation motif to efficiently xylosylate its substrates | ? | - |
- |
|
UDP-alpha-D-xylose + [factor VII protein with EGF-like domain 1]-L-serine | Homo sapiens | - |
UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-xylosyl)-L-serine | - |
? | |
UDP-alpha-D-xylose + [Notch1 protein with EGF-like domain 12]-L-serine | Homo sapiens | - |
UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-xylosyl)-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8NBL1 | - |
- |
Purification (Comment) | Organism |
---|---|
IgG Sepharose affinity chromatography, HiTrap Sepharose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not require a diserine-containing glycosylation motif to efficiently xylosylate its substrates | Homo sapiens | ? | - |
- |
|
UDP-alpha-D-xylose + [factor VII protein with EGF-like domain 1]-L-serine | - |
Homo sapiens | UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-xylosyl)-L-serine | - |
? | |
UDP-alpha-D-xylose + [Notch1 protein with EGF-like domain 12]-L-serine | - |
Homo sapiens | UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-xylosyl)-L-serine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
POGLUT1 | - |
Homo sapiens |
protein-O-glucosyltransferase 1 | - |
Homo sapiens |
Rumi | - |
Homo sapiens |