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Literature summary for 2.4.2.2 extracted from
- Thermophilic nucleoside phosphorylases their properties, characteristics and applications (2020), Biochim. Biophys. Acta, 1868, 140304 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is useful in biocatalytic production of 2,6-dichloropurine 2'-deoxyriboside and 6-chloro-2-fluoropurine 2'-deoxyriboside | Parageobacillus thermoglucosidasius |
| synthesis | the enzyme is useful in biocatalytic production of 2-chloro-2'-deoxyadenosine (cladribine), adenine-arabinoside, 2-fluoroadenine arabinoside, and 5-methyluridine | Geobacillus stearothermophilus |
| synthesis | the enzyme is useful in biocatalytic production of 2-chloroadenine riboside, 2-fluoroadenine riboside, 2,6-diaminopurine 2'-deoxyriboside, 2-chloro-2'-deoxyadenosine, 2-fluoro-2'-deoxyadenosine, 2'-deoxy-2'-fluoroadenosine, 2'-deoxy-2'-fluoro-arabinofuranosyl adenine, 2,6-diaminopurine arabinoside, 2,6-dichloropurine arabinoside, 6-chloro-2-fluoropurine arabinoside, and 5-iododeoxyuridine | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate | Thermus thermophilus | - |
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose | - |
r |  |
| 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate | Geobacillus stearothermophilus | - |
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate | Parageobacillus thermoglucosidasius | - |
uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate | Thermus thermophilus | - |
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate | Geobacillus stearothermophilus | - |
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate | Parageobacillus thermoglucosidasius | - |
uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose | - |
r | |
| 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate | Thermus thermophilus | - |
uracil + 2-deoxy-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate | Geobacillus stearothermophilus | - |
uracil + 2-deoxy-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate | Parageobacillus thermoglucosidasius | - |
uracil + 2-deoxy-beta-D-arabinofuranose | - |
r | |
| 2'-deoxythymidine + phosphate | Thermus thermophilus | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 2'-deoxythymidine + phosphate | Geobacillus stearothermophilus | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 2'-deoxythymidine + phosphate | Parageobacillus thermoglucosidasius | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-thymidine + phosphate | Thermus thermophilus | - |
5-bromo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-thymidine + phosphate | Geobacillus stearothermophilus | - |
5-bromo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-thymidine + phosphate | Parageobacillus thermoglucosidasius | - |
5-bromo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-uridine + phosphate | Thermus thermophilus | - |
5-bromo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-uridine + phosphate | Geobacillus stearothermophilus | - |
5-bromo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-uridine + phosphate | Parageobacillus thermoglucosidasius | - |
5-bromo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-thymidine + phosphate | Thermus thermophilus | - |
5-iodo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-thymidine + phosphate | Geobacillus stearothermophilus | - |
5-iodo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-thymidine + phosphate | Parageobacillus thermoglucosidasius | - |
5-iodo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-uridine + phosphate | Thermus thermophilus | - |
5-iodo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-uridine + phosphate | Geobacillus stearothermophilus | - |
5-iodo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-uridine + phosphate | Parageobacillus thermoglucosidasius | - |
5-iodo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | Thermus thermophilus | - |
5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | Geobacillus stearothermophilus | - |
5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | Parageobacillus thermoglucosidasius | - |
5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | Thermus thermophilus | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | Geobacillus stearothermophilus | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | Parageobacillus thermoglucosidasius | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | Thermus thermophilus | - |
thymine + alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | Geobacillus stearothermophilus | - |
thymine + alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | Parageobacillus thermoglucosidasius | - |
thymine + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | Thermus thermophilus | - |
uracil + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | Geobacillus stearothermophilus | - |
uracil + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | Parageobacillus thermoglucosidasius | - |
uracil + alpha-D-ribose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P39142 | - |
- |
| Bacillus subtilis 168 | P39142 | - |
- |
| Geobacillus stearothermophilus | P77836 | - |
- |
| Parageobacillus thermoglucosidasius | A0A178U4P5 | - |
- |
| Thermus thermophilus | Q72HS4 | - |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the purified enzyme is stable at 50% DMF, 50% DMSO, and 50% 2-MeTHF | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate | - |
Thermus thermophilus | uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate | - |
Geobacillus stearothermophilus | uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-uracil + phosphate | - |
Parageobacillus thermoglucosidasius | uracil + 2-deoxy-2-fluoro-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate | - |
Thermus thermophilus | uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate | - |
Geobacillus stearothermophilus | uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose | - |
r | |
| 2'-deoxy-2'-fluoro-beta-D-ribofuranosyl-uracil + phosphate | - |
Parageobacillus thermoglucosidasius | uracil + 2-deoxy-2-fluoro-beta-D-ribofuranose | - |
r | |
| 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate | - |
Thermus thermophilus | uracil + 2-deoxy-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate | - |
Geobacillus stearothermophilus | uracil + 2-deoxy-beta-D-arabinofuranose | - |
r | |
| 2'-deoxy-beta-D-arabinofuranosyl-uracil + phosphate | - |
Parageobacillus thermoglucosidasius | uracil + 2-deoxy-beta-D-arabinofuranose | - |
r | |
| 2'-deoxythymidine + phosphate | - |
Thermus thermophilus | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 2'-deoxythymidine + phosphate | - |
Geobacillus stearothermophilus | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 2'-deoxythymidine + phosphate | - |
Parageobacillus thermoglucosidasius | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| 2,6-dichloropurine 2'-deoxyriboside + phosphate | - |
Parageobacillus thermoglucosidasius | ? | - |
r | |
| 5-bromo-thymidine + phosphate | - |
Thermus thermophilus | 5-bromo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-thymidine + phosphate | - |
Geobacillus stearothermophilus | 5-bromo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-bromo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-uridine + phosphate | - |
Thermus thermophilus | 5-bromo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-uridine + phosphate | - |
Geobacillus stearothermophilus | 5-bromo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-bromo-uridine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-bromo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-thymidine + phosphate | - |
Thermus thermophilus | 5-iodo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-thymidine + phosphate | - |
Geobacillus stearothermophilus | 5-iodo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-iodo-thymine + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-uridine + phosphate | - |
Thermus thermophilus | 5-iodo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-uridine + phosphate | - |
Geobacillus stearothermophilus | 5-iodo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-iodo-uridine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-iodo-uracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | - |
Thermus thermophilus | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | - |
Geobacillus stearothermophilus | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| 5-methyluridine + phosphate | - |
Parageobacillus thermoglucosidasius | 5-methyluracil + alpha-D-ribose 1-phosphate | - |
r | |
| 6-chloro-2-fluoropurine 2'-deoxyriboside + phosphate | - |
Parageobacillus thermoglucosidasius | ? | - |
r | |
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Thermus thermophilus | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Geobacillus stearothermophilus | ? | - |
- |
|
| additional information | thermophilic PNPs and PyNPs accept nucleosides with arabinose, C2'-fluororibose and C2'-fluoroarabinose as the sugar moiety. Substrate spectrum of thermostable nucleoside phosphorylases, overview | Parageobacillus thermoglucosidasius | ? | - |
- |
|
| thymidine + phosphate | - |
Thermus thermophilus | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | - |
Geobacillus stearothermophilus | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | - |
Thermus thermophilus | thymine + alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | - |
Geobacillus stearothermophilus | thymine + alpha-D-ribose 1-phosphate | - |
r | |
| thymidine + phosphate | - |
Parageobacillus thermoglucosidasius | thymine + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | - |
Thermus thermophilus | uracil + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | - |
Geobacillus stearothermophilus | uracil + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | - |
Parageobacillus thermoglucosidasius | uracil + alpha-D-ribose 1-phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BsPyNP | - |
Bacillus subtilis |
| GsPyNP | - |
Geobacillus stearothermophilus |
| GtPyNP | - |
Parageobacillus thermoglucosidasius |
| PDP | - |
Bacillus subtilis |
| PYNP | - |
Bacillus subtilis |
| PYNP | - |
Thermus thermophilus |
| PYNP | - |
Geobacillus stearothermophilus |
| PYNP | - |
Parageobacillus thermoglucosidasius |
| pyrimidine nucleoside phosphorylase | - |
Bacillus subtilis |
| pyrimidine nucleoside phosphorylase | - |
Thermus thermophilus |
| pyrimidine nucleoside phosphorylase | - |
Geobacillus stearothermophilus |
| pyrimidine nucleoside phosphorylase | - |
Parageobacillus thermoglucosidasius |
| TtPyNP | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Parageobacillus thermoglucosidasius |
| 70 | - |
- |
Geobacillus stearothermophilus |
| 99 | - |
above | Bacillus subtilis |
| 99 | - |
above | Thermus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermophilic signature of the enzyme, overview | Bacillus subtilis |
| additional information | - |
thermophilic signature of the enzyme, overview | Thermus thermophilus |
| additional information | - |
thermophilic signature of the enzyme, overview | Geobacillus stearothermophilus |
| additional information | - |
thermophilic signature of the enzyme, overview | Parageobacillus thermoglucosidasius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified enzyme, half-life is over 16 h | Parageobacillus thermoglucosidasius |
| 70 | - |
purified enzyme, half-life is 1.6 h | Parageobacillus thermoglucosidasius |
| 70 | - |
purified enzyme, half-life is 23 min in the presence of 20 mM potassium phosphate and 1 mM inosine, and 15.1 h in the presence of 20 mM potassium phosphate and 1 mM 5-methyluridine | Geobacillus stearothermophilus |
| 80 | - |
purified enzyme, half-life is over 23 h | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Bacillus subtilis |
| 5 | 6 | - |
Thermus thermophilus |
| 7 | 11.5 | - |
Geobacillus stearothermophilus |
| 7.5 | 8.5 | - |
Parageobacillus thermoglucosidasius |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the NP family II | Thermus thermophilus |
| evolution | the enzyme belongs to the NP family II | Geobacillus stearothermophilus |
| evolution | the enzyme belongs to the NP family II | Parageobacillus thermoglucosidasius |
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