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Literature summary for 2.4.2.17 extracted from

  • Ruszkowski, M.
    Guarding the gateway to histidine biosynthesis in plants Medicago truncatula ATP-phosphoribosyltransferase in relaxed and tense states (2018), Biochem. J., 475, 2681-2697 .
    View publication on PubMed
Search for more literature for 2.4.2.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 gold cells Medicago truncatula

Crystallization (Commentary)

Crystallization (Comment) Organism
native and SeMet-labeled enzyme, hanging drop vapor diffusion method, using 2.53 M NaCl, 100 mM Bis-Tris propane pH 6.3, and 10% (v/v) glycerol Medicago truncatula

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive inhibition Medicago truncatula
L-histidine complete inhibition at 0.1 mM Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.182
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.0 and 24°C Medicago truncatula
0.47
-
 ATP at pH 8.0 and 24°C Medicago truncatula

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Medicago truncatula 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Medicago truncatula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Medicago truncatula
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Medicago truncatula
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Medicago truncatula G7JFL4
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap resin column chromatography and Superdex S200 gel filtration Medicago truncatula

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Medicago truncatula ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Medicago truncatula 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Subunits

Subunits Comment Organism
homohexamer x-ray crystallography Medicago truncatula

Synonyms

Synonyms Comment Organism
ATP-PRT1
-
 Medicago truncatula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.57
-
 ATP at pH 8.0 and 24°C Medicago truncatula
0.57
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.0 and 24°C Medicago truncatula

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0061
-
 in the presence of 2 mM AMP, at pH 8.0 and 24°C Medicago truncatula L-histidine
0.0301
-
 in the absence of AMP, at pH 8.0 and 24°C Medicago truncatula L-histidine

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2
-
 ATP at pH 8.0 and 24°C Medicago truncatula
3.1
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.0 and 24°C Medicago truncatula