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Literature summary for 2.4.2.17 extracted from

  • Cho, Y.; Sharma, V.; Sacchettini, J.C.
    Crystal structure of ATP phosphoribosyltransferase from Mycobacterium tuberculosis (2003), J. Biol. Chem., 278, 8333-8339.
    View publication on PubMed
Search for more literature for 2.4.2.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method at 16°C, the apocrystals are obtained using 0.1 M buffer MES pH 6.5 and magnesium sulfate as precipitant, crystals in the presence of AMP and histidine are obtained using 0.1 M sodium citrate pH 5.6, 0.5 M ammonium sulfate and 1 M lithium sulfate with 5 mM AMP and 0.1 mM histidine Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
L-histidine allosteric inhibition, synergistically favored by AMP; feed-back inhibition Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
 diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid column, Sephadex G-200 later on Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Subunits

Subunits Comment Organism
dimer active form Mycobacterium tuberculosis
hexamer inactive form, in complex with histidine Mycobacterium tuberculosis