Literature summary for 2.4.1.82 extracted from

Tian, C.; Yang, J.; Zeng, Y.; Zhang, T.; Zhou, Y.; Men, Y.; You, C.; Zhu, Y.; Sun, Y.
Biosynthesis of raffinose and stachyose from sucrose via an in vitro multienzyme system (2019), Appl. Environ. Microbiol., 85, e02306 .

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Application

Application	Comment	Organism
synthesis	use of an in vitro multienzyme system using sucrose synthase (SUS), UDP-glucose 4-epimerase (GalE), galactinol synthase (GS), raffinose synthase (RS), and stachyose synthase (STS) and intermedia UDP and inositol, which can be recycled, to synthesize raffinose,. The reaction system produces 11.1mM raffinose using purified enzymes under optimal reaction conditions and substrate concentrations. Optimization further increases raffinose production to 86.6 mM and enables the synthesis of 61.1 mM stachyose. The UDP turnover number reaches 337. Inositol in the reaction system is recycled five times, and 255.8 mM raffinose (128.9 g/liter) is obtained	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9SYJ4	-	-

Synonyms

Synonyms	Comment	Organism
At4g01970	-	Arabidopsis thaliana
RFS4	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)