Crystallization (Comment) | Organism |
---|---|
recombinant enzyme mutant Q345F, in the presence of sucrose, and in complex with glucose, X-ray diffraction structure determination and analysis at 2.7 A resolution, PDB ID 5C8B | Bifidobacterium adolescentis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | redesign of the active site of sucrose phosphorylase through a clash-induced cascade of loop shifts | Bifidobacterium adolescentis |
Q345F | site-directed mutagenesis, the mutation allows efficient glucosylation of resveratrol, (+)-catechin and (-)-epicatechin in yields of up to 97% whereas the wild-type enzyme favours sucrose hydrolysis. The crystal structure of the variant reveals a widened access channel with a hydrophobic aromatic surface that is likely to contribute to the improved activity towards aromatic acceptors. The generation of this channel can be explained in terms of a cascade of structural changes arising from the Q345F exchange, structural changes in the active site of BaSP Q345F, modeling, overview | Bifidobacterium adolescentis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + phosphate | Bifidobacterium adolescentis | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | Q84HQ2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the wild-type enzyme shows poor activity with flavonoids or stilbenoids, e.g. resveratrol, (+)-catechin and (-)-epicatechin, while the compounds are substrates of the enzyme mutant Q345F | Bifidobacterium adolescentis | ? | - |
? | |
sucrose + (+)-catechin | activity of enzyme mutant Q345F | Bifidobacterium adolescentis | D-fructose + (+)-catechin 3'-O-alpha-D-glucoside + (+)-catechin 3',5-O-alpha-D-diglucoside | - |
? | |
sucrose + (-)-epicatechin | activity of enzyme mutant Q345F | Bifidobacterium adolescentis | D-fructose + (-)-epicatechin 3'-O-alpha-D-glucoside + (-)-epicatechin 5-O-alpha-D-glucoside + (-)-epicatechin 3',5-O-alpha-D-diglucoside | - |
? | |
sucrose + phosphate | - |
Bifidobacterium adolescentis | D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
sucrose + phosphate | activity of wild-type enzyme and mutant Q345F | Bifidobacterium adolescentis | D-fructose + alpha-D-glucose 1-phosphate | - |
r | |
sucrose + resveratrol | activity of enzyme mutant Q345F | Bifidobacterium adolescentis | D-fructose + resveratrol 3-O-alpha-D-glucoside | - |
? |