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Literature summary for 2.4.1.68 extracted from

  • Ihara, H.; Okada, T.; Ikeda, Y.
    Cloning, expression and characterization of Bombyx mori alpha1,6-fucosyltransferase (2014), Biochem. Biophys. Res. Commun., 450, 953-960.
    View publication on PubMed
Search for more literature for 2.4.1.68

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf21 insect cells Bombyx mori

Protein Variants

Protein Variants Comment Organism
C46A the mutant shows wild type activity Bombyx mori
N183/341Q the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
N183D the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
N183D/N341D the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
N183Q the mutant shows wild type activity Bombyx mori
N341D the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
N341Q the mutant shows reduced activity compared to the wild type enzyme Bombyx mori
R359A inactive Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 2 * 66000, SDS-PAGE Bombyx mori
120000
-
 gel filtration Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-beta-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine Bombyx mori
-
 GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori A0A060PLD2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein Asn-183 and Asn-341 are the consensus sites for N-glycosylation Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Bombyx mori GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 66000, SDS-PAGE Bombyx mori

Synonyms

Synonyms Comment Organism
alpha1,6-fucosyltransferase
-
 Bombyx mori
alpha1,6FucT
-
 Bombyx mori