Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 20% inhibition at 1 mM | Pediococcus pentosaceus | |
additional information | no effect by 7 M urea on enzyme activity | Pediococcus pentosaceus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pediococcus pentosaceus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | activates 150% at 6 mM | Pediococcus pentosaceus | |
MgCl2 | activates 5% at 1 mM | Pediococcus pentosaceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
native PAGE | Pediococcus pentosaceus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + [(1->6)-alpha-D-glucosyl]n | Pediococcus pentosaceus | - |
D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | at 50%, reduces the enzyme activity by 94% | Pediococcus pentosaceus |
acetonitrile | at 20%, reduces the enzyme activity by 80% | Pediococcus pentosaceus |
DMSO | at 90%, reduces the enzyme activity by 91% | Pediococcus pentosaceus |
Ethanol | at 50%, reduces the enzyme activity by 80% | Pediococcus pentosaceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pediococcus pentosaceus | - |
isolated from soil from a sugarcane field in Assam, India | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme by a single-step fractionation using polyethylene glycols of different molecular mass, 31fold purification by PEG 400 and 45fold purification by PEG 1500 | Pediococcus pentosaceus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
crude enzyme, culture supernatant, pH 5.4, 30°C | Pediococcus pentosaceus |
18 | - |
PEG 400-purified enzyme, pH 5.4, 30°C | Pediococcus pentosaceus |
36 | - |
PEG 1500-purified enzyme, pH 5.4, 30°C | Pediococcus pentosaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | optimization of conditions for dextransucrase activity assay | Pediococcus pentosaceus | ? | - |
? | |
sucrose + [(1->6)-alpha-D-glucosyl]n | - |
Pediococcus pentosaceus | D-fructose + [(1->6)-alpha-D-glucosyl]n+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pediococcus pentosaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Pediococcus pentosaceus |