Cloned (Comment) | Organism |
---|---|
gene dsrF, DNA and amino acid sequence determination and analysis, sequence comparison, expression of DSR-F in Escherichia coli strain JM109 | Leuconostoc citreum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a truncated active variant DSR-F-DELTASPDELTAGBD of 1251 amino acids, with a molecular mass of 145544 Da, the mutant lacks the sequence encoding signal peptide and a portion of the C-terminal domain, i.e. the glucan binding domain | Leuconostoc citreum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | DSR-F C-terminal glucan binding domain might be involved in enzyme anchoring at the cell wall surface | Leuconostoc citreum | 5618 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170448 | - |
x * 170448, DSRF, sequence calculation | Leuconostoc citreum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Leuconostoc citreum | dextransucrase DSR-F from Leuconostoc citreum B/110-1-2 is a novel sucrose glucosyltransferase specific for alpha-1,6 and alpha-1,3 glucosidic bond synthesis, with alpha-1,4 branching | ? | - |
? | |
additional information | Leuconostoc citreum B/110-1-2 | dextransucrase DSR-F from Leuconostoc citreum B/110-1-2 is a novel sucrose glucosyltransferase specific for alpha-1,6 and alpha-1,3 glucosidic bond synthesis, with alpha-1,4 branching | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc citreum | D5FS20 | - |
- |
Leuconostoc citreum B/110-1-2 | D5FS20 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | dextransucrase DSR-F from Leuconostoc citreum B/110-1-2 is a novel sucrose glucosyltransferase specific for alpha-1,6 and alpha-1,3 glucosidic bond synthesis, with alpha-1,4 branching | Leuconostoc citreum | ? | - |
? | |
additional information | the recombinant enzyme catalyzes oligosaccharides synthesis from sucrose as donor and maltose acceptor | Leuconostoc citreum | ? | - |
? | |
additional information | dextransucrase DSR-F from Leuconostoc citreum B/110-1-2 is a novel sucrose glucosyltransferase specific for alpha-1,6 and alpha-1,3 glucosidic bond synthesis, with alpha-1,4 branching | Leuconostoc citreum B/110-1-2 | ? | - |
? | |
additional information | the recombinant enzyme catalyzes oligosaccharides synthesis from sucrose as donor and maltose acceptor | Leuconostoc citreum B/110-1-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 170448, DSRF, sequence calculation | Leuconostoc citreum |
More | the C-terminus, also named glucan binding domain, GBD, begins at position 1171 and is 358 residues in size, the DSR-F C-terminal GBD also contains the unusual repeats named APY 2 times | Leuconostoc citreum |
Synonyms | Comment | Organism |
---|---|---|
DSR-F | - |
Leuconostoc citreum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leuconostoc citreum | sequence calculation | - |
5.26 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside-hydrolase family GH70, transglucosidases that produce a-glucans from sucrose | Leuconostoc citreum |
additional information | amino acid residues D557, E595, and D667 are forming the catalytic triad | Leuconostoc citreum |