General Stability | Organism |
---|---|
irreversible inactivation in absence of Triton X-100 | Sus scrofa |
loss of activity after freezing in buffer of pH 7.5 | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UMP | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, sedimentation equilibrium of reduced and carboxymethylated enzyme | Sus scrofa |
100000 | - |
zonal centrifugation, gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Sus scrofa |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
submaxillary gland | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-70°C, 0.025 M MES buffer, pH 6.0, 1 mM EDTA, 0.03% Triton X-100, after removal of most of the buffer by dialysis, stable for 1 year | Sus scrofa |
4°C, pH 7.5, half-life is about 3 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + asialo-porcine submaxillary mucin of A-negative blood-type | - |
Sus scrofa | UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[asialo-porcine submaxillary mucin of A-negative blood-type] | - |
? | |
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose | - |
Sus scrofa | UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, sedimentation equilibrium of reduced and carboxymethylated enzyme | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Sus scrofa |