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Literature summary for 2.4.1.364 extracted from

  • Jung, S.; Kim, W.; Park, S.; Jeong, J.; Park, M.; Lim, S.; Lee, Y.; Im, W.; Lee, J.; Choi, G.; Kim, S.
    Two ginseng UDP-glycosyltransferases synthesize ginsenoside Rg 3 and Rd (2014), Plant Cell Physiol., 55, 2177-2188 .
    View publication on PubMed
Search for more literature for 2.4.1.364

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells and in Saccharomyces cerevisiae Panax ginseng

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 (20S)-protopanaxadiol at pH 8.0 and 35°C Panax ginseng
0.04
-
 ginsenoside C-K at pH 8.0 and 35°C Panax ginseng

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + (20S)-protopanaxadiol Panax ginseng
-
 UDP + (20S)-ginsenoside Rh2
-
 ?
UDP-alpha-D-glucose + ginsenoside C-K Panax ginseng
-
 UDP + ginsenoside F2
-
 ?

Organism

Organism UniProt Comment Textmining
Panax ginseng A0A0A6ZFR4
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Panax ginseng

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Panax ginseng
-
root highest expression Panax ginseng
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme glucosylates the C3 position of both (20S)-protopanaxadiol and ginsenoside C-K, but not C20 or C12 positions and also does not glucosylate hydroxyl residues at C3, C6, C12 or C20 positions in any protopanaxadiol-type ginsenoside Panax ginseng ?
-
 ?
UDP-alpha-D-glucose + (20S)-protopanaxadiol
-
 Panax ginseng UDP + (20S)-ginsenoside Rh2
-
 ?
UDP-alpha-D-glucose + ginsenoside C-K
-
 Panax ginseng UDP + ginsenoside F2
-
 ?

Synonyms

Synonyms Comment Organism
UGT74AE2
-
 Panax ginseng

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000556
-
 (20S)-protopanaxadiol at pH 8.0 and 35°C Panax ginseng
0.0001347
-
 ginsenoside C-K at pH 8.0 and 35°C Panax ginseng

General Information

General Information Comment Organism
metabolism key enzyme for the synthesis of ginsenosides Panax ginseng

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00224
-
 (20S)-protopanaxadiol at pH 8.0 and 35°C Panax ginseng
0.00337
-
 ginsenoside C-K at pH 8.0 and 35°C Panax ginseng