Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli SoluBL21 cells | Streptomyces venezuelae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
0.44 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
0.9 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
0.93 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
109000 | - |
gel filtration | Streptomyces venezuelae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-glucose + glucose 6-phosphate | Streptomyces venezuelae | - |
GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-glucose + glucose 6-phosphate | Streptomyces venezuelae ATCC 10712 | - |
GDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | F2RH01 | - |
- |
Streptomyces venezuelae ATCC 10712 | F2RH01 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography and Superdex 200 gel filtration | Streptomyces venezuelae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
ADP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae ATCC 10712 | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-glucose + glucose 6-phosphate | the enzyme has a preference for GDP-glucose | Streptomyces venezuelae | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae ATCC 10712 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-glucose + glucose 6-phosphate | the enzyme has a preference for GDP-glucose | Streptomyces venezuelae ATCC 10712 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 51000, calculated from amino acid sequence | Streptomyces venezuelae |
Synonyms | Comment | Organism |
---|---|---|
alpha,alpha-trehalose-phosphate synthase | - |
Streptomyces venezuelae |
OtsA | - |
Streptomyces venezuelae |
trehalose-6-phosphate synthase | - |
Streptomyces venezuelae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
29 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
123 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
139 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
67 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
132 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
1143 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae |