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Literature summary for 2.4.1.25 extracted from

  • Kaper, T.; Talik, B.; Ettema, T.J.; Bos, H.; van der Maarel, M.J.; Dijkhuizen, L.
    Amylomaltase of Pyrobaculum aerophilum IM2 produces thermoreversible starch gels (2005), Appl. Environ. Microbiol., 71, 5098-5106.
    View publication on PubMed
Search for more literature for 2.4.1.25

Application

Application Comment Organism
nutrition acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties. This thermoreversible gel has potential applications in the food industry Pyrobaculum aerophilum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrobaculum aerophilum

General Stability

General Stability Organism
dithiothreitol decreases thermal stability of the enzyme Pyrobaculum aerophilum

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
 Pyrobaculum aerophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 maltoheptaose pH 6.7, 80°C Pyrobaculum aerophilum
2.1
-
 maltotetraose pH 6.7, 80°C Pyrobaculum aerophilum
3.2
-
 maltohexaose pH 6.7, 80°C Pyrobaculum aerophilum
3.7
-
 maltotriose pH 6.7, 80°C Pyrobaculum aerophilum
6.9
-
 maltopentaose pH 6.7, 80°C Pyrobaculum aerophilum

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum
-
 IM2
-
Pyrobaculum aerophilum IM2
-
 IM2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + maltoheptaose
-
 Pyrobaculum aerophilum D-glucose + maltooligosaccharides
-
 ?
maltoheptaose + maltoheptaose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltooligosaccharides
-
 ?
maltohexaose + maltohexaose
-
 Pyrobaculum aerophilum D-glucose + maltooligosaccharides
-
 ?
maltohexaose + maltohexaose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltooligosaccharides
-
 ?
maltopentaose + maltopentaose
-
 Pyrobaculum aerophilum D-glucose + maltooligosaccharides
-
 ?
maltopentaose + maltopentaose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltooligosaccharides
-
 ?
maltotetraose + maltotetraose
-
 Pyrobaculum aerophilum D-glucose + maltooligosaccharides
-
 ?
maltotetraose + maltotetraose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltooligosaccharides
-
 ?
maltotriose + maltotriose
-
 Pyrobaculum aerophilum D-glucose + maltopentaose
-
 ?
maltotriose + maltotriose
-
 Pyrobaculum aerophilum IM2 D-glucose + maltopentaose
-
 ?
additional information acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties Pyrobaculum aerophilum ?
-
 ?

Synonyms

Synonyms Comment Organism
amylmaltase
-
 Pyrobaculum aerophilum
PyAMase
-
 Pyrobaculum aerophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
 Pyrobaculum aerophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
87
-
 half-life: 506 min Pyrobaculum aerophilum
89
-
 half-life: 114 min Pyrobaculum aerophilum
95
-
 half-life: 107 min Pyrobaculum aerophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.7
-
 maltoheptaose pH 6.7, 80°C Pyrobaculum aerophilum
8.9
-
 maltohexaose pH 6.7, 80°C Pyrobaculum aerophilum
24.8
-
 maltopentaose pH 6.7, 80°C Pyrobaculum aerophilum
51.2
-
 maltotetraose pH 6.7, 80°C Pyrobaculum aerophilum
115
-
 maltotriose pH 6.7, 80°C Pyrobaculum aerophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Pyrobaculum aerophilum