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Literature summary for 2.4.1.25 extracted from
- 4-alpha-glucanotransferase from the hyperthermophilic archaeon Thermococcus litoralis. Enzyme purification and characterization, and gene cloning, sequencing and expression in Escherichia coli (1997), Eur. J. Biochem., 248, 171-178.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cloned an sequenced | Dictyoglomus thermophilum |
| Gtase gene cloned and expressed in Escherichia coli MV1184 | Thermococcus litoralis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Thermococcus litoralis |  |
| Monoiodoacetic acid | - |
Thermococcus litoralis | |
| Ni2+ | - |
Thermococcus litoralis | |
| p-chloromercuribenzoic acid | - |
Thermococcus litoralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 77880 | - |
calculated from cDNA | Thermococcus litoralis |
| 85000 | - |
SDS-PAGE | Thermococcus litoralis |
| 87000 | - |
gel filtration | Thermococcus litoralis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Dictyoglomus thermophilum | - |
- |
- |
| Pyrococcus furiosus | - |
- |
- |
| Solanum tuberosum | - |
potato | - |
| Streptococcus mitis | - |
- |
- |
| Thermococcus litoralis | - |
DSM 5473 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus litoralis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
| leaf | - |
Solanum tuberosum | - |
| tuber | - |
Arabidopsis thaliana | - |
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Thermococcus litoralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + D-glucose | - |
Solanum tuberosum | low molecular mass oligosaccharides | - |
? | |
| amylose + D-glucose | synthetic amylose AS-320 | Thermococcus litoralis | low molecular mass oligosaccharides | - |
? | |
| amylose + maltopentaose | - |
Solanum tuberosum | cyclic alpha-1,4-glucan | cycloamylose | ? | |
| amylose + maltopentaose | - |
Thermococcus litoralis | cyclic alpha-1,4-glucan | cycloamylose | ? | |
| maltoheptaose + maltopentaose | - |
Arabidopsis thaliana | maltooligosaccharides | - |
? | |
| maltoheptaose + maltopentaose | - |
Thermococcus litoralis | maltooligosaccharides | - |
? | |
| maltohexaose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
| maltooligosaccharides | - |
Thermococcus litoralis | maltooligosaccharides + D-glucose | - |
? | |
| maltopentaose + maltopentaose | - |
Thermococcus litoralis | new oligosaccharides | - |
? | |
| maltose + maltose | - |
Thermococcus litoralis | maltotriose + glucose | - |
? | |
| maltotetraose + maltopentaose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| maltotetraose + maltopentaose | - |
Arabidopsis thaliana | maltooligosaccharides | - |
? | |
| maltotetraose + maltopentaose | - |
Thermococcus litoralis | maltooligosaccharides | - |
? | |
| maltotheptaose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
| maltotriose + glycosyl acceptor | - |
Pyrococcus furiosus | D-glucose + maltose + maltotetraose + maltopentaose + maltohexaose | - |
? | |
| maltotriose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
| maltotriose + maltotriose | - |
Thermococcus litoralis | maltopentaose + maltotetraose | - |
? | |
| additional information | - |
Arabidopsis thaliana | ? | - |
? | |
| additional information | enzyme catalyzes not only intermolecular transglycosylation to produce linear alpha-1,4-glucan, but also intramolecular transglycosylation to produce cyclic alpha-1,4-glucan | Thermococcus litoralis | ? | - |
? | |
| additional information | maltose is not a product | Solanum tuberosum | ? | - |
? | |
| starch + D-glucose | - |
Solanum tuberosum | low molecular mass oligosaccharides | - |
? | |
| starch + D-glucose | soluble starch | Thermococcus litoralis | low molecular mass oligosaccharides | - |
? | |
| starch + glycosyl acceptor | - |
Pyrococcus furiosus | D-glucose + maltose + glucan oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Solanum tuberosum |
| monomer | 1 * 85000, SDS-PAGE | Thermococcus litoralis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Thermococcus litoralis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 100 | - |
Thermococcus litoralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
quite thermostable, retains full activity after 3 h | Thermococcus litoralis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermococcus litoralis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10 | completely stable at 4°C for 18 h | Thermococcus litoralis |
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